ArticleNature protocols2011
Fluorescent in situ visualization of sterols in Arabidopsis roots.
Article in Nature protocols, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- Advances in mass spectrometry of lipids for the investigation of Niemann-pick type C disease.Lipids in health and disease · 2025Review
- Sterols in plant biologyCell surface (Amsterdam, Netherlands) · 2025Review
- Root expansion microscopy: A robust method for super resolution imaging in Arabidopsis.The Plant cell · 2025Article
- Imaging Plant Lipids with Fluorescent Reporters.Plants (Basel, Switzerland) · 2024Review
- Phytosterols reverse antiretroviral-induced hearing loss, with potential implications for cochlear aging.PLoS biology · 2023Article
- The Sterol Trafficking Pathway inFrontiers in plant science · 2021Article
- Tools of the trade: studying actin in zebrafish.Histochemistry and cell biology · 2020Review
- Auxin-induced signaling protein nanoclustering contributes to cell polarity formation.Nature communications · 2020Article
- Ethylene and hydrogen peroxide regulate formation of a sterol-enriched domain essential for wall labyrinth assembly in transfer cells.Journal of experimental botany · 2019Article
- Identification of Parameters of Host Cell Vulnerability during Salmonella Infection by Quantitative Image Analysis and Modeling.Infection and immunity · 2018Article
- Label-free visualization of fruit lignification: Raman molecular imaging of loquat lignified cells.Plant methods · 2018Article
- Clathrin inFrontiers in plant science · 2017Article
- Proliferation and Morphogenesis of the Endoplasmic Reticulum Driven by the Membrane Domain of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase in Plant Cells.Plant physiology · 2015Article
- Loss of the Arabidopsis thaliana P4-ATPases ALA6 and ALA7 impairs pollen fitness and alters the pollen tube plasma membrane.Frontiers in plant science · 2015Article
- Mapping of Membrane Lipid Order in Root Apex Zones of Arabidopsis thaliana.Frontiers in plant science · 2015Article
- High lipid order of Arabidopsis cell-plate membranes mediated by sterol and DYNAMIN-RELATED PROTEIN1A function.The Plant journal : for cell and molecular biology · 2014Article
- Visualization of cholesterol deposits in lysosomes of Niemann-Pick type C fibroblasts using recombinant perfringolysin O.Orphanet journal of rare diseases · 2014Article
- Sterol-dependent induction of plant defense responses by a microbe-associated molecular pattern from Trichoderma viride.Plant physiology · 2014Article
- The endoplasmic reticulum is the main membrane source for biogenesis of the lytic vacuole in Arabidopsis.The Plant cell · 2013Article
- Visualization of sterol-rich membrane domains with fluorescently-labeled theonellamides.PloS one · 2013Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sterols are eukaryotic membrane components with crucial roles in diverse cellular processes. Elucidation of sterol function relies on development of tools for in situ sterol visualization. Here we describe protocols for in situ sterol localization in Arabidopsis thaliana root cells, using filipin as a specific probe for detection of fluorescent filipin-sterol complexes. Currently, filipin is the only established tool for sterol visualization in plants. Filipin labeling can be performed on aldehyde-fixed samples, largely preserving fluorescent proteins and being compatible with immunocytochemistry. Filipin can also be applied for probing live cells, taking into account the fact that it inhibits sterol-dependent endocytosis. The experimental procedures described are designed for fluorescence detection by confocal laser-scanning microscopy with excitation of filipin-sterol complexes at 364 nm. The protocols require 1 d for sterol covisualization with fluorescent proteins in fixed or live roots and 2 d for immunocytochemistry on whole-mount roots.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.