ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2011
FACS identifies unique cocaine-induced gene regulation in selectively activated adult striatal neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 89 citations in OpenAlex.
- Neuronal Grin2c expression and the cocaine engram: a medial prefrontal cortex substrate for conditioned place preference.bioRxiv : the preprint server for biology · 2026Article
- Drug-Related Engrams and Their Role in the Persistence and Recurrence of Drug-Related Behaviors.Hippocampus · 2025Review
- Cocaine-context memories are transcriptionally encoded in nucleus accumbens Arc ensembles.Nature communications · 2025Article
- Cocaine-induced gene regulation in D1 and D2 neuronal ensembles of the nucleus accumbens.Communications biology · 2025Article
- Reelin marks cocaine-activated striatal neurons, promotes neuronal excitability, and regulates cocaine reward.Science advances · 2025Article
- Profiling gene alterations in striatonigral neurons associated with incubation of methamphetamine craving by cholera toxin subunit B-based fluorescence-activated cell sorting.Frontiers in cellular neuroscience · 2025Article
- Relapse after electric barrier-induced voluntary abstinence: A review.Current opinion in neurobiology · 2024Review
- Temporally specific gene expression and chromatin remodeling programs regulate a conservedeLife · 2023Article
- Advancing the preclinical study of comorbid neuroHIV and substance use disorders: Current perspectives and future directions.Brain, behavior, and immunity · 2023Review
- MultipleXed Population Selection and Enrichment single nucleus RNA sequencing (XPoSE-seq) enables sample identity retention during transcriptional profiling of rare populations.bioRxiv : the preprint server for biology · 2023Article
- Flow Cytometry of Synaptoneurosomes (FCS) Reveals Increased Ribosomal S6 and Calcineurin Proteins in Activated Medial Prefrontal Cortex to Nucleus Accumbens Synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Role of Piriform Cortex and Its Afferent Projections in Relapse to Fentanyl Seeking after Food Choice-Induced Voluntary Abstinence.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Role of ventral subiculum neuronal ensembles in incubation of oxycodone craving after electric barrier-induced voluntary abstinence.Science advances · 2023Article
- Exploring the memory: existing activity-dependent tools to tag and manipulate engram cells.Frontiers in cellular neuroscience · 2023Review
- Isolation of living dopaminergic neurons labeled with a fluorescent ligand of the dopamine transporter from mouse substantia nigra as a new tool for basic and applied research.Frontiers in molecular neuroscience · 2022Article
- HDAC3 Activity within the Nucleus Accumbens Regulates Cocaine-Induced Plasticity and Behavior in a Cell-Type-Specific Manner.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Article
- Drug-activated cells: From immediate early genes to neuronal ensembles in addiction.Advances in pharmacology (San Diego, Calif.) · 2021Review
- Genetic Architecture and Molecular Neuropathology of Human Cocaine Addiction.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- A dopamine-induced gene expression signature regulates neuronal function and cocaine response.Science advances · 2020Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Numerous studies with the neural activity marker Fos indicate that cocaine activates only a small proportion of sparsely distributed striatal neurons. Until now, efficient methods were not available to assess neuroadaptations induced specifically within these activated neurons. We used fluorescence-activated cell sorting (FACS) to purify striatal neurons activated during cocaine-induced locomotion in naive and cocaine-sensitized cfos-lacZ transgenic rats. Activated neurons were labeled with an antibody against β-galactosidase, the protein product of the lacZ gene. Cocaine induced a unique gene expression profile selectively in the small proportion of activated neurons that was not observed in the nonactivated majority of neurons. These genes included altered levels of the immediate early genes arc, fosB, and nr4a3, as well as genes involved in p38 MAPK signaling and cell-type specificity. We propose that this FACS method can be used to study molecular neuroadaptations in specific neurons encoding the behavioral effects of abused drugs and other learned behaviors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.