ArticleMolecular biology of the cell2011
Clathrin-dependent mechanisms modulate the subcellular distribution of class C Vps/HOPS tether subunits in polarized and nonpolarized cells.
Article in Molecular biology of the cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- AP-3 vesicle uncoating occurs after HOPS-dependent vacuole tethering.The EMBO journal · 2020Article
- Mechanism of platelet α-granule biogenesis: study of cargo transport and the VPS33B-VPS16B complex in a model system.Blood advances · 2019Article
- Adaptor protein-3 complex is required for Vangl2 trafficking and planar cell polarity of the inner ear.Molecular biology of the cell · 2019Article
- Coat/Tether Interactions-Exception or Rule?Frontiers in cell and developmental biology · 2016Review
- Caenorhabditis elegans HOPS and CCZ-1 mediate trafficking to lysosome-related organelles independently of RAB-7 and SAND-1.Molecular biology of the cell · 2014Article
- Photocleavable dimerizer for the rapid reversal of molecular trap antagonists.The Journal of biological chemistry · 2014Article
- Vps33b pathogenic mutations preferentially affect VIPAS39/SPE-39-positive endosomes.Human molecular genetics · 2013Article
- Presynaptic membrane retrieval and endosome biology: defining molecularly heterogeneous synaptic vesicles.Cold Spring Harbor perspectives in biology · 2013Review
- Chemical-genetic disruption of clathrin function spares adaptor complex 3-dependent endosome vesicle biogenesis.Molecular biology of the cell · 2013Article
- Notch signaling from the endosome requires a conserved dileucine motif.Molecular biology of the cell · 2013Article
- Associations among genotype, clinical phenotype, and intracellular localization of trafficking proteins in ARC syndrome.Human mutation · 2012Article
- Molecular architecture of the multisubunit homotypic fusion and vacuole protein sorting (HOPS) tethering complex.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Article
- Metazoan cell biology of the HOPS tethering complex.Cellular logistics · 2011Article
- The Vps39-like TRAP1 is an effector of Rab5 and likely the missing Vps3 subunit of human CORVET.Cellular logisticsArticle
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Coats define the composition of carriers budding from organelles. In addition, coats interact with membrane tethers required for vesicular fusion. The yeast AP-3 (Adaptor Protein Complex 3) coat and the class C Vps/HOPS (HOmotypic fusion and Protein Sorting) tether follow this model as their interaction occurs at the carrier fusion step. Here we show that mammalian Vps class C/HOPS subunits and clathrin interact and that acute perturbation of clathrin function disrupts the endosomal distribution of Vps class C/HOPS tethers in HEK293T and polarized neuronal cells. Vps class C/HOPS subunits and clathrin exist in complex with either AP-3 or hepatocyte growth factor receptor substrate (Hrs). Moreover, Vps class C/HOPS proteins cofractionate with clathrin-coated vesicles, which are devoid of Hrs. Expression of FK506 binding protein (FKBP)-clathrin light chain chimeras, to inhibit clathrin membrane association dynamics, increased Vps class C/HOPS subunit content in rab5 endosomal compartments. Additionally, Vps class C/HOPS subunits were concentrated at tips of neuronal processes, and their delivery was impaired by expression of FKBP-clathrin chimeras and AP20187 incubation. These data support a model in which Vps class C/HOPS subunits incorporate into clathrin-coated endosomal domains and carriers in mammalian cells. We propose that vesicular (AP-3) and nonvesicular (Hrs) clathrin mechanisms segregate class C Vps/HOPS tethers to organelles and domains of mammalian cells bearing complex architectures.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.