ArticleBMC genomics2011
Sequential analysis of global gene expression profiles in immature and in vitro matured bovine oocytes: potential molecular markers of oocyte maturation.
Article in BMC genomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 93 citations in OpenAlex.
- Overview of Gene Expression Dynamics during Human Oogenesis/Folliculogenesis.International journal of molecular sciences · 2023Pooled it
- Exploration of the dynamic regulation of the hypothalamic-pituitary-ovarian axis during the estrous cycle.Journal of ovarian research · 2026Article
- Kinglet in the Poultry Court of Russia: Whole-Genome Insights into Ancestry, Genetic Variability, Selection Footprints and Candidate Genes in a Unique Local Chicken Breed Relative to Other Bantam/Dwarf Breeds.Animals : an open access journal from MDPI · 2026Article
- When bovine life begins in a petri dish: transcriptomic insights from embryos to calves conceived in vitro.The Journal of reproduction and development · 2026Review
- Sequential miRNA-mRNA profiling of regulatory pathways during in vitro maturation of canine oocytes.Animal reproduction · 2026Article
- Single-cell multiomic analysis reveals methylome and transcriptome deviations following oocyte maturation in vitro.Reproduction (Cambridge, England) · 2025Article
- The dominant follicle: the final frontier in bovine oocyte development.Animal reproduction · 2025Article
- Meiotic maturation failure in primary ovarian insufficiency: insights from a bovine model.Journal of assisted reproduction and genetics · 2024Article
- Combining genome-wide association study and transcriptome analysis to identify molecular markers and genetic basis of population-asynchronous ovarian development inZoological research · 2024Article
- TXNL4B regulates radioresistance by controlling the PRP3-mediated alternative splicing of FANCI.MedComm · 2023Article
- Using Cumulus Cell Biopsy as a Non-Invasive Tool to Access the Quality of Bovine Oocytes: How Informative Are They?Animals : an open access journal from MDPI · 2022Article
- Identifying novel genetic loci associated with polycystic ovary syndrome based on its shared genetic architecture with type 2 diabetes.Frontiers in genetics · 2022Article
- Transcriptome and proteome analysis of pregnancy and postpartum anoestrus ovaries in yak.Journal of veterinary science · 2022Article
- Cellular Processes in Human Ovarian Follicles Are Regulated by Expression Profile of New Gene Markers-Clinical Approach.Journal of clinical medicine · 2021Article
- Single-Cell RNA-Seq Revealed the Gene Expression Pattern during the In Vitro Maturation of Donkey Oocytes.Genes · 2021Article
- Article
- STAT3 signaling stimulates miR-21 expression in bovine cumulus cells during in vitro oocyte maturation.Scientific reports · 2018Article
- Use of Confocal Microscopy to Evaluate Equine Zygote Development After Sperm Injection of Oocytes Matured In Vivo or In Vitro.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2017Article
- Ovarian transcriptomic analysis of Shan Ma ducks at peak and late stages of egg production.Asian-Australasian journal of animal sciences · 2017Article
- Identification of potential biomarkers in donor cows for in vitro embryo production by granulosa cell transcriptomics.PloS one · 2017Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWithout intensive selection, the majority of bovine oocytes submitted to in vitro embryo production (IVP) fail to develop to the blastocyst stage. This is attributed partly to their maturation status and competences. Using the Affymetrix GeneChip Bovine Genome Array, global mRNA expression analysis of immature (GV) and in vitro matured (IVM) bovine oocytes was carried out to characterize the transcriptome of bovine oocytes and then use a variety of approaches to determine whether the observed transcriptional changes during IVM was real or an artifact of the techniques used during analysis.
results8489 transcripts were detected across the two oocyte groups, of which ~25.0% (2117 transcripts) were differentially expressed (p < 0.001); corresponding to 589 over-expressed and 1528 under-expressed transcripts in the IVM oocytes compared to their immature counterparts. Over expression of transcripts by IVM oocytes is particularly interesting, therefore, a variety of approaches were employed to determine whether the observed transcriptional changes during IVM were real or an artifact of the techniques used during analysis, including the analysis of transcript abundance in oocytes in vitro matured in the presence of α-amanitin. Subsets of the differentially expressed genes were also validated by quantitative real-time PCR (qPCR) and the gene expression data was classified according to gene ontology and pathway enrichment. Numerous cell cycle linked (CDC2, CDK5, CDK8, HSPA2, MAPK14, TXNL4B), molecular transport (STX5, STX17, SEC22A, SEC22B), and differentiation (NACA) related genes were found to be among the several over-expressed transcripts in GV oocytes compared to the matured counterparts, while ANXA1, PLAU, STC1and LUM were among the over-expressed genes after oocyte maturation.
conclusionUsing sequential experiments, we have shown and confirmed transcriptional changes during oocyte maturation. This dataset provides a unique reference resource for studies concerned with the molecular mechanisms controlling oocyte meiotic maturation in cattle, addresses the existing conflicting issue of transcription during meiotic maturation and contributes to the global goal of improving assisted reproductive technology.
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Registered trials
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