ArticleMolecular microbiology2011
Expression of the ompATb operon accelerates ammonia secretion and adaptation of Mycobacterium tuberculosis to acidic environments.
Article in Molecular microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed, 56 citations in OpenAlex.
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- Acidic pH Restricts Non-Tuberculous Mycobacteria Replication.Molecular microbiology · 2026Article
- STK-mediated FadR phosphorylation regulates the acid resistance and virulence of Streptococcus suis.PLoS pathogens · 2025Article
- Unraveling Mycobacterium tuberculosis acid resistance and pH homeostasis mechanisms.FEBS letters · 2025Review
- Isolation and characterization of a protective monoclonal antibody targeting outer membrane protein (OmpA) against tuberculosis.Microbiology spectrum · 2025Article
- Exploring the Potential of Exosomes as Biomarkers in Tuberculosis and Other Diseases.International journal of molecular sciences · 2024Review
- Molecular Identification and Acid Stress Response of anInternational journal of molecular sciences · 2023Article
- Comparative Proteomic Analysis Revealing ActJ-Regulated Proteins inJournal of proteome research · 2023Article
- The Role of Amino Acids in Tuberculosis Infection: A Literature Review.Metabolites · 2022Review
- Phenotypic adaptation ofFrontiers in cellular and infection microbiology · 2022Review
- Production of ammonia makes Venusian clouds habitable and explains observed cloud-level chemical anomalies.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- AC2P20 selectively killsRSC advances · 2021Article
- Transporters Involved in the Biogenesis and Functionalization of the Mycobacterial Cell Envelope.Chemical reviews · 2021Review
- De novo histidine biosynthesis protects Mycobacterium tuberculosis from host IFN-γ mediated histidine starvation.Communications biology · 2021Article
- Vaccines Against Tuberculosis: Problems and Prospects (Review).Applied biochemistry and microbiology · 2020Article
- Determinants of Phagosomal pH During Host-Pathogen Interactions.Frontiers in cell and developmental biology · 2020Review
- Acid Fasting: Modulation of Mycobacterium tuberculosis Metabolism at Acidic pH.Trends in microbiology · 2019Review
- Unraveling the role of the transcriptional regulator VirS in low pH-induced responses ofThe Journal of biological chemistry · 2019Article
- Candida albicans Hyphal Expansion Causes Phagosomal Membrane Damage and Luminal Alkalinization.mBio · 2018Article
- Amino Acids As Mediators of Metabolic Cross Talk between Host and Pathogen.Frontiers in immunology · 2018Review
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Authors and funding
8 authors at 2 institutions in 1 country.
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Abstract
Homeostasis of intracellular pH is a trait critical for survival of Mycobacterium tuberculosis in macrophages. However, mechanisms by which M. tuberculosis adapts to acidic environments are poorly understood. In this study, we analysed the physiological functions of OmpATb, a surface-accessible protein of M. tuberculosis. OmpATb did not complement the permeability defects of a Mycobacterium smegmatis porin mutant to glucose, serine and glycerol, in contrast to the porin MspA. Uptake rates of these solutes were unchanged in an ompATb operon mutant of M. tuberculosis indicating that OmpATb is not a general porin. Chemical analysis of low-pH culture filtrates showed that the proteins encoded by the ompATb operon are involved in generating a rapid ammonia burst, which neutralized medium pH and preceded exponential growth of M. tuberculosis. Addition of ammonia accelerated growth of the ompATb operon mutant demonstrating that ammonia secretion is indeed a mechanism by which M. tuberculosis neutralizes acidic environments. Infection experiments revealed that the ompATb operon was not required for full virulence in mice suggesting that M. tuberculosis has multiple mechanisms of resisting phagosomal acidification. Taken together, these results show that the ompATb operon is necessary for rapid ammonia secretion and adaptation of M. tuberculosis to acidic environments in vitro but not in mice.
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