ArticleAnalytical chemistry2011
Nine orders of magnitude dynamic range: picomolar to millimolar concentration measurement in capillary electrophoresis with laser induced fluorescence detection employing cascaded avalanche photodiode photon counters.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Article
- 3-D printed injection system for capillary electrophoresis.Analytical methods : advancing methods and applications · 2022Article
- Article
- Space- and time-resolved investigation on diffusion kinetics of human skin following macromolecule delivery by microneedle arrays.Scientific reports · 2018Article
- A Low-Cost Palmtop High-Speed Capillary Electrophoresis Bioanalyzer with Laser Induced Fluorescence Detection.Scientific reports · 2018Article
- Capillary Electrophoresis with Laser-Induced Fluorescent Detection of Immunolabeled Individual Autophagy Organelles Isolated from Liver Tissue.Analytical chemistry · 2016Article
- Fluorescently labelled glycans and their applications.Glycoconjugate journal · 2015Review
- Article
- Small-volume analysis of cell-cell signaling molecules in the brain.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2014Review
- Simplified sheath flow cuvette design for ultrasensitive laser induced fluorescence detection in capillary electrophoresis.The Analyst · 2012Article
- Single cell ganglioside catabolism in primary cerebellar neurons and glia.Neurochemical research · 2012Article
- Statistical connection of binomial photon counting and photon averaging in high dynamic range beam-scanning microscopy.Optics express · 2012Article
- Metabolic cytometry: capillary electrophoresis with two-color fluorescence detection for the simultaneous study of two glycosphingolipid metabolic pathways in single primary neurons.Analytical chemistry · 2012Article
- Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The dynamic range of capillary electrophoresis analysis is ultimately limited by molecular shot noise at low concentrations and by concentration-induced band broadening at high concentrations. We report a system that approaches these fundamental limits. A laser-induced fluorescence detector is reported that employs a cascade of four fiber-optic beam splitters connected in series to generate a primary signal and four attenuated signals, each monitored by a single-photon counting avalanche photodiode. Appropriate scaling of the signals from the five photodiodes produces a linear optical calibration curve for 5-carboxyl-tetramethylrhodamine from the concentration detection limit of 1 pM to the upper limit of 1 mM. Mass detection limits are 120 yoctomoles (70 molecules) injected into the instrument. The very-wide dynamic range instrument was used to study the metabolic products of the fluorescently labeled glycosphingolipid tetramethylrhodamine labeled GM1 (GM1-TMR) produced by single cells isolated from the rat cerebellum.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.