Evidence map›Paper›PMID 21406073›Full record

ArticleBiological procedures online2010

Utilization of IκB-EGFP Chimeric Gene as an Indicator to Identify Microbial Metabolites with NF-κB Inhibitor Activity.

Yu-Ling Lin, Yen-Shun Chen, Jui-Hung Hsieh, Ching-Min Lin, Hsin-Yi Wu, Chen-Si Lin, Rea-Min Chu, Kuang-Wen Liao, Yuan-Hsun Hsu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biological procedures online, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 87% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Yu-Ling Lin *Institute of Molecular Medicine and Bioengineering, National Chiao Tung University, Hsinchu, Taiwan.
Yen-Shun Chen *Graduate Institute of Medical Sciences, Taipei Medical University, Taipei, Taiwan.
Jui-Hung HsiehDepartment of Electronics Engineering and Institute of Electronics, National Chiao Tung University, Hsinchu, Taiwan.
Ching-Min LinInstitute of Molecular Medicine and Bioengineering, National Chiao Tung University, Hsinchu, Taiwan.
Hsin-Yi WuInstitute of Molecular Medicine and Bioengineering, National Chiao Tung University, Hsinchu, Taiwan.
Chen-Si LinAnimal Cancer Center, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
Rea-Min ChuAnimal Cancer Center, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
Kuang-Wen LiaoInstitute of Molecular Medicine and Bioengineering, National Chiao Tung University, Hsinchu, Taiwan.
Yuan-Hsun HsuDepartment of Microbiology and Immunology, College of Medicine, Taipei Medical University, Taipei, Taiwan.
National Yang Ming Chiao Tung University · TWNational Taiwan University · TWTaipei Medical University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

NF-κB regulates several important expressions, such as cytokine release, anti-apoptosis, adhesion molecule expression, and cell cycle processing. Several NF-κB inhibitors have been discovered as an anti-tumor or anti-inflammatory drug. The activity of NF-κB transcription factor is negatively regulated by IκB binding. In this study, IκB assay system was established and IκB-EGFP fusion protein was used as an indicator to monitor the effects of substances on the IκB degradation. The results indicated that the chosen hydroquinone could inhibit the IκB degradation and cause the cell de-attachment from the bottom of culture plate. In addition, this system could also monitor the IκB degradation of microbial metabolite of natural mixtures of propolis. Thus, the IκB assay system may be a good system for drug discovery related to microbial metabolite.

Identifiers

PMID21406073
PMCPMC3055915
OpenAlexW2154681134

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.