ArticleBiological procedures online2009
Ocular proteomics with emphasis on two-dimensional gel electrophoresis and mass spectrometry.
Article in Biological procedures online, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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Who cites it
13 citing papers in PubMed.
- Alternative Splicing Regulation in Metabolic Disorders.Obesity reviews : an official journal of the International Association for the Study of Obesity · 2025Review
- Vitreous Biomarkers for Proliferative Vitreoretinopathy Prognostication in Patients Undergoing Primary Retinal Detachment Repair.Translational vision science & technology · 2024Article
- Mass spectrometry analysis of human tear fluid biomarkers specific for ocular and systemic diseases in the context of 3P medicine.The EPMA journal · 2021Review
- Minocycline reduces inflammatory response and cell death in a S100B retina degeneration model.Journal of neuroinflammation · 2020Article
- Protein Biomarkers in Uveitis.Frontiers in immunology · 2020Review
- In-Depth Proteomic Analysis of the Porcine Retina by Use of a four Step Differential Extraction Bottom up LC MS Platform.Molecular neurobiology · 2017Article
- A Review: Proteomics in Retinal Artery Occlusion, Retinal Vein Occlusion, Diabetic Retinopathy and Acquired Macular Disorders.International journal of molecular sciences · 2017Review
- Proteomic Analysis of Various Rat Ocular Tissues after Ischemia-Reperfusion Injury and Possible Relevance to Acute Glaucoma.International journal of molecular sciences · 2017Article
- Proteomic Analysis of the Vitreous following Experimental Retinal Detachment in Rabbits.Journal of ophthalmology · 2015Article
- Dye-Free Porcine Model of Experimental Branch Retinal Vein Occlusion: A Suitable Approach for Retinal Proteomics.Journal of ophthalmology · 2015Article
- A proteomics view of the molecular mechanisms and biomarkers of glaucomatous neurodegeneration.Progress in retinal and eye research · 2013Review
- Proteomic analyses of the vitreous humour.Mediators of inflammation · 2012Article
- Protein changes in the retina following experimental retinal detachment in rabbits.Molecular vision · 2011Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The intention of this review is to provide an overview of current methodologies employed in the rapidly developing field of ocular proteomics with emphasis on sample preparation, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry (MS). Appropriate sample preparation for the diverse range of cells and tissues of the eye is essential to ensure reliable results. Current methods of protein staining for 2D-PAGE, protein labelling for two-dimensional difference gel electrophoresis, gel-based expression analysis and protein identification by MS are summarised. The uses of gel-free MS-based strategies (MuDPIT, iTRAQ, ICAT and SILAC) are also discussed. Proteomic technologies promise to shed new light onto ocular disease processes that could lead to the discovery of strong novel biomarkers and therapeutic targets useful in many ophthalmic conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.