ArticleBiotechnology and bioengineering2011
In situ metabolic flux analysis to quantify the liver metabolic response to experimental burn injury.
Article in Biotechnology and bioengineering, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
- Study on metabolic disorders in rat liver induced by different times after scalds.Biochemistry and biophysics reports · 2025Article
- Systemic long-term metabolic effects of acute non-severe paediatric burn injury.Scientific reports · 2022Article
- Cutaneous Thermal Injury Modulates Blood and Skin Metabolomes Differently in a Murine Model.Journal of burn care & research : official publication of the American Burn Association · 2021Article
- Metabolic Modelling as a Framework for Metabolomics Data Integration and Analysis.Metabolites · 2020Review
- Mechanistic identification of biofluid metabolite changes as markers of acetaminophen-induced liver toxicity in rats.Toxicology and applied pharmacology · 2019Article
- Metabolic network-based predictions of toxicant-induced metabolite changes in the laboratory rat.Scientific reports · 2018Article
- Nuclear receptors control pro-viral and antiviral metabolic responses to hepatitis C virus infection.Nature chemical biology · 2016Article
- Kupffer Cell p38 Mitogen-Activated Protein Kinase Signaling Drives Postburn Hepatic Damage and Pulmonary Inflammation When Alcohol Intoxication Precedes Burn Injury.Critical care medicine · 2016Article
- Burn injury induces histopathological changes and cell proliferation in liver of rats.World journal of hepatology · 2016Article
- Article
- SIMPLE MACHINE PERFUSION SIGNIFICANTLY ENHANCES HEPATOCYTE YIELDS OF ISCHEMIC AND FRESH RAT LIVERS.Cell medicine · 2013Article
- Article
- Resuscitation of ischemic donor livers with normothermic machine perfusion: a metabolic flux analysis of treatment in rats.PloS one · 2013Article
- Development of Metabolic Indicators of Burn Injury: Very Low Density Lipoprotein (VLDL) and Acetoacetate Are Highly Correlated to Severity of Burn Injury in Rats.Metabolites · 2012Article
- Subnormothermic machine perfusion at both 20°C and 30°C recovers ischemic rat livers for successful transplantation.The Journal of surgical research · 2012Article
- A simplified subnormothermic machine perfusion system restores ischemically damaged liver grafts in a rat model of orthotopic liver transplantation.Transplantation research · 2012Article
- Metabolic response of perfused livers to various oxygenation conditions.Biotechnology and bioengineering · 2011Article
- Advanced stoichiometric analysis of metabolic networks of mammalian systems.Critical reviews in biomedical engineering · 2011Review
- Alcohol Modulation of the Postburn Hepatic Response.Journal of burn care & research : official publication of the American Burn AssociationReview
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Trauma such as burns induces a hypermetabolic response associated with altered central carbon and nitrogen metabolism. The liver plays a key role in these metabolic changes; however, studies to date have evaluated the metabolic state of liver using ex vivo perfusions or isotope labeling techniques targeted to specific pathways. Herein, we developed a unique mass balance approach to characterize the metabolic state of the liver in situ, and used it to quantify the metabolic changes to experimental burn injury in rats. Rats received a sham (control uninjured), 20% or 40% total body surface area (TBSA) scald burn, and were allowed to develop a hypermetabolic response. One day prior to evaluation, all animals were fasted to deplete glycogen stores. Four days post-burn, blood flow rates in major vessels of the liver were measured, and blood samples harvested. We combined measurements of metabolite concentrations and flow rates in the major vessels entering and leaving the liver with a steady-state mass balance model to generate a quantitative picture of the metabolic state of liver. The main findings were: (1) Sham-burned animals exhibited a gluconeogenic pattern, consistent with the fasted state; (2) the 20% TBSA burn inhibited gluconeogenesis and exhibited glycolytic-like features with very few other significant changes; (3) the 40% TBSA burn, by contrast, further enhanced gluconeogenesis and also increased amino acid extraction, urea cycle reactions, and several reactions involved in oxidative phosphorylation. These results suggest that increasing the severity of injury does not lead to a simple dose-dependent metabolic response, but rather leads to qualitatively different responses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.