ArticleInfection and immunity2011
Dictyostelium discoideum as a model system for identification of Burkholderia pseudomallei virulence factors.
Article in Infection and immunity, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 44 citations in OpenAlex.
- Article
- Marmosets as models of infectious diseases.Frontiers in cellular and infection microbiology · 2024Review
- Studies of Streptococcus anginosus Virulence in Dictyostelium discoideum and Galleria mellonella Models.Infection and immunity · 2023Article
- Article
- Characterizing the Type 6 Secretion System (T6SS) and its role in the virulence of avian pathogenicPeerJ · 2021Article
- Regulation of Virulence by Two-Component Systems in PathogenicInfection and immunity · 2020Review
- Distinct Pathogenic Patterns ofThe American journal of tropical medicine and hygiene · 2019Article
- PIKfyve/Fab1 is required for efficient V-ATPase and hydrolase delivery to phagosomes, phagosomal killing, and restriction of Legionella infection.PLoS pathogens · 2019Article
- The Mechanisms of Disease Caused byFrontiers in microbiology · 2019Review
- Inorganic Polyphosphate Is Essential forFrontiers in cellular and infection microbiology · 2018Article
- Diversity of Free-Living Environmental Bacteria and Their Interactions With a Bactivorous Amoeba.Frontiers in cellular and infection microbiology · 2018Article
- Comparative analysis of the Burkholderia cenocepacia K56-2 essential genome reveals cell envelope functions that are uniquely required for survival in species of the genus Burkholderia.Microbial genomics · 2017Article
- Dissection of Francisella-Host Cell Interactions in Dictyostelium discoideum.Applied and environmental microbiology · 2015Article
- Melioidosis: molecular aspects of pathogenesis.Expert review of anti-infective therapy · 2014Review
- Cross-species comparison of the Burkholderia pseudomallei, Burkholderia thailandensis, and Burkholderia mallei quorum-sensing regulons.Journal of bacteriology · 2014Article
- Characterization and analysis of the Burkholderia pseudomallei BsaN virulence regulon.BMC microbiology · 2014Article
- Global analysis of the Burkholderia thailandensis quorum sensing-controlled regulon.Journal of bacteriology · 2014Article
- Article
- Article
- Bacterial discrimination by dictyostelid amoebae reveals the complexity of ancient interspecies interactions.Current biology : CB · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Burkholderia pseudomallei is an emerging bacterial pathogen and category B biothreat. Human infections with B. pseudomallei (called melioidosis) present as a range of manifestations, including acute septicemia and pneumonia. Although melioidosis can be fatal, little is known about the molecular basis of B. pseudomallei pathogenicity, in part because of the lack of simple, genetically tractable eukaryotic models to facilitate en masse identification of virulence determinants or explore host-pathogen interactions. Two assays, one high-throughput and one quantitative, were developed to monitor levels of resistance of B. pseudomallei and the closely related nearly avirulent species Burkholderia thailandensis to predation by the phagocytic amoeba Dictyostelium discoideum. The quantitative assay showed that levels of resistance to, and survival within, amoeba by these bacteria and their known virulence mutants correlate well with their published levels of virulence in animals. Using the high-throughput assay, we screened a 1,500-member B. thailandensis transposon mutant library and identified 13 genes involved in resistance to predation by D. discoideum. Orthologs of these genes were disrupted in B. pseudomallei, and nearly all mutants had similarly decreased resistance to predation by D. discoideum. For some mutants, decreased resistance also correlated with reduced survival in and cytotoxicity toward macrophages, as well as attenuated virulence in mice. These observations suggest that some factors required by B. pseudomallei for resistance to environmental phagocytes also aid in resistance to phagocytic immune cells and contribute to disease in animals. Thus, D. discoideum provides a novel, high-throughput model system for facilitating inquiry into B. pseudomallei virulence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.