ArticleInfection and immunity2011
Regulation of virulence by the RevR response regulator in Clostridium perfringens.
Article in Infection and immunity, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Detection ofVirulence · 2025Article
- Molecular identification of hyaluronate lyase, not hyaluronidase, as an intrinsic hyaluronan-degrading enzyme in Clostridium perfringens strain ATCC 13124.Scientific reports · 2024Article
- The biology and pathogenicity ofMicrobiology and molecular biology reviews : MMBR · 2024Review
- Potent Bile Acid Microbial Metabolites ModulatePathogens (Basel, Switzerland) · 2023Article
- Pathogenicity and virulence ofVirulence · 2021Article
- Sialidases FromFrontiers in cellular and infection microbiology · 2019Review
- Article
- NanR RegulatesInfection and immunity · 2017Article
- Clostridium perfringens Sialidases: Potential Contributors to Intestinal Pathogenesis and Therapeutic Targets.Toxins · 2016Review
- Review
- RNA-seq analysis of virR and revR mutants of Clostridium perfringens.BMC genomics · 2016Article
- Transcriptional Profile during Deoxycholate-Induced Sporulation in a Clostridium perfringens Isolate Causing Foodborne Illness.Applied and environmental microbiology · 2016Article
- Animal models to study the pathogenesis of human and animal Clostridium perfringens infections.Veterinary microbiology · 2015Review
- Article
- Utility of the clostridial site-specific recombinase TnpX to clone toxic-product-encoding genes and selectively remove genomic DNA fragments.Applied and environmental microbiology · 2014Article
- Comparative transcription analysis and toxin production of two fluoroquinolone-resistant mutants of Clostridium perfringens.BMC microbiology · 2013Article
- Regulation of sialidase production in Clostridium perfringens by the orphan sensor histidine kinase ReeS.PloS one · 2013Article
- Genome sequencing and analysis of a type A Clostridium perfringens isolate from a case of bovine clostridial abomasitis.PloS one · 2012Article
- A novel toxin regulator, the CPE1446-CPE1447 protein heteromeric complex, controls toxin genes in Clostridium perfringens.Journal of bacteriology · 2011Article
- Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clostridium perfringens causes clostridial myonecrosis or gas gangrene and produces several extracellular hydrolytic enzymes and toxins, many of which are regulated by the VirSR signal transduction system. The revR gene encodes a putative orphan response regulator that has similarity to the YycF (WalR), VicR, PhoB, and PhoP proteins from other Gram-positive bacteria. RevR appears to be a classical response regulator, with an N-terminal receiver domain and a C-terminal domain with a putative winged helix-turn-helix DNA binding region. To determine its functional role, a revR mutant was constructed by allelic exchange and compared to the wild type by microarray analysis. The results showed that more than 100 genes were differentially expressed in the mutant, including several genes involved in cell wall metabolism. The revR mutant had an altered cellular morphology; unlike the short rods observed with the wild type, the mutant cells formed long filaments. These changes were reversed upon complementation with a plasmid that carried the wild-type revR gene. Several genes encoding extracellular hydrolytic enzymes (sialidase, hyaluronidase, and α-clostripain) were differentially expressed in the revR mutant. Quantitative enzyme assays confirmed that these changes led to altered enzyme activity and that complementation restored the wild-type phenotype. Most importantly, the revR mutant was attenuated for virulence in the mouse myonecrosis model compared to the wild type and the complemented strains. These results provide evidence that RevR regulates virulence in C. perfringens; it is the first response regulator other than VirR to be shown to regulate virulence in this important pathogen.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.