ArticleBiophysical journal2011
On the mechanical stabilization of filopodia.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Engineering 3D Scaffold-Free Nanoparticle-Laden Stem Cell Constructs for Piezoelectric Enhancement of Human Neural Tissue Formation and Function.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- How filopodia respond to calcium in the absence of a calcium-binding structural protein: non-channel functions of TRP.Cell communication and signaling : CCS · 2022Article
- Filopodia rotate and coil by actively generating twist in their actin shaft.Nature communications · 2022Article
- Force-exerting perpendicular lateral protrusions in fibroblastic cell contraction.Communications biology · 2020Article
- Registry Kinetics of Myosin Motor Stacks Driven by Mechanical Force-Induced Actin Turnover.Biophysical journal · 2019Article
- Macropinosome formation by tent pole ruffling in macrophages.The Journal of cell biology · 2018Article
- Measuring Local Viscosities near Plasma Membranes of Living Cells with Photonic Force Microscopy.Biophysical journal · 2015Article
- Surfing along Filopodia: A Particle Transport Revealed by Molecular-Scale Fluctuation Analyses.Biophysical journal · 2015Article
- Promoting filopodial elongation in neurons by membrane-bound magnetic nanoparticles.Nanomedicine : nanotechnology, biology, and medicine · 2015Article
- Helical buckling of actin inside filopodia generates traction.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Physical model for the geometry of actin-based cellular protrusions.Biophysical journal · 2014Article
- Physics of cell adhesion: some lessons from cell-mimetic systems.Soft matter · 2014Review
- Filopodial retraction force is generated by cortical actin dynamics and controlled by reversible tethering at the tip.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The sense is in the fingertips: The distal end controls filopodial mechanics and dynamics in response to external stimuli.Communicative & integrative biology · 2013Article
- Differential dynamics of platelet contact and spreading.Biophysical journal · 2012Article
- Cell cytoskeleton and tether extraction.Biophysical journal · 2011Article
- Dynamic buckling of actin within filopodia.Communicative & integrative biologyArticle
- Protein fluxes along the filopodium as a framework for understanding the growth-retraction dynamics: the interplay between diffusion and active transport.Cell adhesion & migrationArticle
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
We studied force-induced elongation of filopodia by coupling magnetic tweezers to the tip through the bacterial coat protein invasin, which couples the force generator to the actin bundles (through myosin X), thus impeding the growth of the actin plus end. Single force pulses (15-30 s) with amplitudes between 20 and 600 pN and staircase-like force scenarios (amplitudes, ∼50 pN; step widths, 30 s) were applied. In both cases, the responses consist of a fast viscoelastic deflection followed by a linear flow regime. The deflections are reversible after switching off the forces, suggesting a mechanical memory. The elongation velocity exhibits an exponential distribution (half-width <v(1/2)>, ∼0.02 μm s(-1)) and did not increase systematically with the force amplitudes. We estimate the bending modulus (0.4 × 10(-23) J m) and the number of actin filaments (∼10) by analyzing filopodium bending fluctuations. Sequestering of intracellular Ca(2+) by BAPTA caused a strong reduction in the amplitude of elongation, whereas latrunculin A resulted in loss of the elastic response. We attribute the force-independent velocity to the elongation of actin bundles enabled by the force-induced actin membrane uncoupling and the reversibility by the treadmilling mechanism and an elastic response.
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