ArticleBiophysical journal2011
Keratocyte lamellipodial protrusion is characterized by a concave force-velocity relation.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 69 citations in OpenAlex.
- Emergence of multiple collective motility modes in a physical model of cell chains.NPJ systems biology and applications · 2025Article
- Mesenchymal cell migration on one-dimensional micropatterns.Frontiers in cell and developmental biology · 2024Review
- On multistability and constitutive relations of cell motion on fibronectin lanes.Biophysical journal · 2023Article
- Membrane Ruffling is a Mechanosensor of Extracellular Fluid Viscosity.Nature physics · 2022Article
- WASp triggers mechanosensitive actin patches to facilitate immune cell migration in dense tissues.Developmental cell · 2022Article
- Discrete mechanical model of lamellipodial actin network implements molecular clutch mechanism and generates arcs and microspikes.PLoS computational biology · 2021Article
- On the adhesion-velocity relation and length adaptation of motile cells on stepped fibronectin lanes.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Lamellipodium tip actin barbed ends serve as a force sensor.Genes to cells : devoted to molecular & cellular mechanisms · 2019Article
- The Cytoskeleton-A Complex Interacting Meshwork.Cells · 2019Review
- Adaptive F-Actin Polymerization and Localized ATP Production Drive Basement Membrane Invasion in the Absence of MMPs.Developmental cell · 2019Article
- Influence of cross-linking and retrograde flow on formation and dynamics of lamellipodium.PloS one · 2019Article
- Building a dendritic actin filament network branch by branch: models of filament orientation pattern and force generation in lamellipodia.Biophysical reviews · 2018Review
- On the relation between filament density, force generation, and protrusion rate in mesenchymal cell motility.Molecular biology of the cell · 2018Article
- Cell protrusion and retraction driven by fluctuations in actin polymerization: A two-dimensional model.Cytoskeleton (Hoboken, N.J.) · 2017Article
- Mechanosensitive Adhesion Explains Stepping Motility in Amoeboid Cells.Biophysical journal · 2017Article
- Power transduction of actin filaments ratcheting in vitro against a load.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Feedback mechanisms in a mechanical model of cell polarization.Physical biology · 2014Article
- Control of actin-based motility through localized actin binding.Physical biology · 2013Article
- Branching and capping determine the force-velocity relationships of branching actin networks.Physical biology · 2013Article
- Finding the weakest link: exploring integrin-mediated mechanical molecular pathways.Journal of cell science · 2012Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report on the characterization of actin driven lamellipodial protrusion forces and velocities in keratocytes. A vertically mounted glass fiber acted as a flexible barrier positioned in front of migrating keratocytes with parallel phase contrast microscopy. A laser beam was coupled into the fiber and allowed detecting the position of the fiber by a segmented photodiode. Calibration of the fiber was carried out with the thermal oscillation method. Deflection and force signals were measured during lamellipodial protrusion. Velocity was constant during initial contact whereas loading force increased until finally the cell was stalled at higher forces. Stall forces were on the order of 2.9 ± 0.6 nN, which corresponds to a stall pressure of 2.7 ± 1.6 nN/μm(2). Assuming a density of actin filaments of 240 filaments per μm, we can estimate a stall force per actin filament of 1.7 ± 0.8 pN. To check for adaption of the cell against an external force, we let the cell push toward the glass fiber several times. On the timescale of the experiment (∼1 min), however, the cell did not adapt to previous loading events.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.