ArticleMalaria journal2011
PvRON2, a new Plasmodium vivax rhoptry neck antigen.
Article in Malaria journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 27 citations in OpenAlex.
- Different clinical forms of Plasmodium vivax infection result in unique T cell epitope-specific immune responses.Journal of immunology (Baltimore, Md. : 1950) · 2026Article
- Characterization of merozoite-specific thrombospondin-related anonymous protein (MTRAP) inFrontiers in cellular and infection microbiology · 2024Article
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- Complement Receptor 1 availability on red blood cell surface modulates Plasmodium vivax invasion of human reticulocytes.Scientific reports · 2019Article
- Genetic sequence characterization and naturally acquired immune response to Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2).Malaria journal · 2018Article
- TheFrontiers in cellular and infection microbiology · 2018Article
- On the Evolution and Function ofFrontiers in genetics · 2018Article
- PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis.Parasites & vectors · 2017Article
- Molecular Cloning and Characterization ofJournal of parasitology research · 2017Article
- Merozoite surface proteins in red blood cell invasion, immunity and vaccines against malaria.FEMS microbiology reviews · 2016Review
- Antigenicity and immunogenicity of PvRALP1, a novel Plasmodium vivax rhoptry neck protein.Malaria journal · 2015Article
- The Plasmodium vivax rhoptry neck protein 5 is expressed in the apical pole of Plasmodium vivax VCG-1 strain schizonts and binds to human reticulocytes.Malaria journal · 2015Article
- Characterization of Plasmodium vivax Early Transcribed Membrane Protein 11.2 and Exported Protein 1.PloS one · 2015Article
- Annotation and characterization of the Plasmodium vivax rhoptry neck protein 4 (PvRON4).Malaria journal · 2013Article
- Characterizing PvARP, a novel Plasmodium vivax antigen.Malaria journal · 2013Article
- Identification, characterization and antigenicity of the Plasmodium vivax rhoptry neck protein 1 (PvRON1).Malaria journal · 2011Article
- Article
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Authors and funding
4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundRhoptries are specialized organelles from parasites belonging to the phylum Apicomplexa; they secrete their protein content during invasion of host target cells and are sorted into discrete subcompartments within rhoptry neck or bulb. This distribution is associated with these proteins' role in tight junction (TJ) and parasitophorous vacuole (PV) formation, respectively.
methodsPlasmodium falciparum RON2 amino acid sequence was used as bait for screening the codifying gene for the homologous protein in the Plasmodium vivax genome. Gene synteny, as well as identity and similarity values, were determined for ron2 and its flanking genes among P. falciparum, P. vivax and other malarial parasite genomes available at PlasmoDB and Sanger Institute databases. Pvron2 gene transcription was determined by RT-PCR of cDNA obtained from the P. vivax VCG-1 strain. Protein expression and localization were assessed by Western blot and immunofluorescence using polyclonal anti-PvRON2 antibodies. Co-localization was confirmed using antibodies directed towards specific microneme and rhoptry neck proteins. RESULTS AND DISCUSSION: The first P. vivax rhoptry neck protein (named here PvRON2) has been identified in this study. PvRON2 is a 2,204 residue-long protein encoded by a single 6,615 bp exon containing a hydrophobic signal sequence towards the amino-terminus, a transmembrane domain towards the carboxy-terminus and two coiled coil α-helical motifs; these are characteristic features of several previously described vaccine candidates against malaria. This protein also contains two tandem repeats within the interspecies variable sequence possibly involved in evading a host's immune system. PvRON2 is expressed in late schizonts and localized in rhoptry necks similar to what has been reported for PfRON2, which suggests its participation during target cell invasion.
conclusionsThe identification and partial characterization of the first P. vivax rhoptry neck protein are described in the present study. This protein is homologous to PfRON2 which has previously been shown to be associated with PfAMA-1, suggesting a similar role for PvRON2.
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