ArticleMolecular microbiology2011
Multiple orphan histidine kinases interact directly with Spo0A to control the initiation of endospore formation in Clostridium acetobutylicum.
Article in Molecular microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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60 citing papers in PubMed, 136 citations in OpenAlex.
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- Identification of serine/threonine kinases that regulate metabolism and sporulation in Clostridium beijerinckii.Applied microbiology and biotechnology · 2022Article
- Kinase expression enhances phenolic aldehydes conversion and ethanol fermentability of Zymomonas mobilis.Bioprocess and biosystems engineering · 2022Article
- Identification of Functional Spo0A Residues Critical for Sporulation in Clostridioides difficile.Journal of molecular biology · 2022Article
- Three Orphan Histidine Kinases Inhibit Clostridioides difficile Sporulation.Journal of bacteriology · 2022Article
- Genetic mechanisms governing sporulation initiation in Clostridioides difficile.Current opinion in microbiology · 2022Review
- Mechanisms and Applications of Bacterial Sporulation and Germination in the Intestine.International journal of molecular sciences · 2022Review
- Agr Quorum Sensing influences the Wood-Ljungdahl pathway in Clostridium autoethanogenum.Scientific reports · 2022Article
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- Epigallocatechin gallate and Lactobacillus plantarum culture supernatants exert bactericidal activity and reduce biofilm formation in Clostridium perfringens.Folia microbiologica · 2021Article
- Sporulation in solventogenic and acetogenic clostridia.Applied microbiology and biotechnology · 2021Review
- CD25890, a conserved protein that modulates sporulation initiation in Clostridioides difficile.Scientific reports · 2021Article
- Quantitative proteomic analysis to reveal expression differences for butanol production from glycerol and glucose by Clostridium sp. strain CT7.Microbial cell factories · 2021Article
- Structural, Metabolic and Evolutionary Comparison of Bacterial Endospore and Exospore Formation.Frontiers in microbiology · 2021Review
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7 authors at 4 institutions in 2 countries.
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Abstract
The phosphorylated Spo0A transcription factor controls the initiation of endospore formation in Clostridium acetobutylicum, but genes encoding key phosphorelay components, Spo0F and Spo0B, are missing in the genome. We hypothesized that the five orphan histidine kinases of C. acetobutylicum interact directly with Spo0A to control its phosphorylation state. Sequential targeted gene disruption and gene expression profiling provided evidence for two pathways for Spo0A activation, one dependent on a histidine kinase encoded by cac0323, the other on both histidine kinases encoded by cac0903 and cac3319. Purified Cac0903 and Cac3319 kinases autophosphorylated and transferred phosphoryl groups to Spo0A in vitro, confirming their role in Spo0A activation in vivo. A cac0437 mutant hyper-sporulated, suggesting that Cac0437 is a modulator that prevents sporulation and maintains cellular Spo0A∼P homeostasis during growth. Accordingly, Cac0437 has apparently lost the ability to autophosphorylate in vitro; instead it catalyses the ATP-dependent dephosphorylation of Spo0A∼P releasing inorganic phosphate. Direct phosphorylation of Spo0A by histidine kinases and dephosphorylation by kinase-like proteins may be a common feature of the clostridia that may represent the ancestral state before the great oxygen event some 2.4 billion years ago, after which additional phosphorelay proteins were recruited in the evolutionary lineage that led to the bacilli.
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