ArticlePlant cell reports2011
Agrobacterium-mediated transformation of kabocha squash (Cucurbita moschata Duch) induced by wounding with aluminum borate whiskers.
Article in Plant cell reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Transgenic Arabidopsis thaliana plants expressing bacterial γ-hexachlorocyclohexane dehydrochlorinase LinA.BMC biotechnology · 2024Article
- Plant biomacromolecule delivery methods in the 21st century.Frontiers in genome editing · 2022Review
- A simple and efficient agroinfiltration method for transient gene expression in Citrus.Plant cell reports · 2021Article
- A method of transformation and current progress in transgenic research on cucumbers andPlant biotechnology (Tokyo, Japan) · 2020Article
- A protocol forPlant biotechnology (Tokyo, Japan) · 2020Article
- Expression of PumpkinFrontiers in plant science · 2020Article
- Improved Powdery Mildew Resistance of TransgenicFrontiers in plant science · 2019Article
- Biodegradation of γ-hexachlorocyclohexane by transgenic hairy root cultures of Cucurbita moschata that accumulate recombinant bacterial LinA.Plant cell reports · 2016Article
- Improvement ofPlant biotechnology reports · 2013Article
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5 authors.
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Abstract
An efficient genetic transformation method for kabocha squash (Cucurbita moschata Duch cv. Heiankogiku) was established by wounding cotyledonary node explants with aluminum borate whiskers prior to inoculation with Agrobacterium. Adventitious shoots were induced from only the proximal regions of the cotyledonary nodes and were most efficiently induced on Murashige-Skoog agar medium with 1 mg/L benzyladenine. Vortexing with 1% (w/v) aluminum borate whiskers significantly increased Agrobacterium infection efficiency in the proximal region of the explants. Transgenic plants were screened at the T(0) generation by sGFP fluorescence, genomic PCR, and Southern blot analyses. These transgenic plants grew normally and T(1) seeds were obtained. We confirmed stable integration of the transgene and its inheritance in T(1) generation plants by sGFP fluorescence and genomic PCR analyses. The average transgenic efficiency for producing kabocha squashes with our method was about 2.7%, a value sufficient for practical use.
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