ArticleParasitology research2011
Use of PCR to detect Entamoeba gingivalis in diseased gingival pockets and demonstrate its absence in healthy gingival sites.
Article in Parasitology research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.
- RETRACTED: Association ofMedicina (Kaunas, Lithuania) · 2024Pooled it
- The association between Trichomonas tenax and Entamoeba gingivalis and periimplantitis and periodontitis.Clinical oral investigations · 2026Article
- The Taxonomy of the GenusPathogens (Basel, Switzerland) · 2026Review
- Beyond Bacteria: The Impact of Protozoa on Periodontal Health.Microorganisms · 2025Review
- Parasitological and molecular study ofArchives of Razi Institute · 2024Article
- Pilot Study on the Prevalence ofMicroorganisms · 2023Article
- First molecular detection of Entamoeba gingivalis subtypes in individuals from Turkey.Pathogens and disease · 2023Article
- Widespread, human-associated redondoviruses infect the commensal protozoan Entamoeba gingivalis.Cell host & microbe · 2023Article
- Article
- Article
- Formulation, Optimization, and Evaluation of Oregano Oil Nanoemulsions for the Treatment of Infections Due to Oral Microbiota.International journal of nanomedicine · 2021Article
- Oral Colonization byFrontiers in cellular and infection microbiology · 2021Article
- Amoebae in Chronic, Polymicrobial Endodontic Infections Are Associated with Altered Microbial Communities of Increased Virulence.Journal of clinical medicine · 2020Article
- Prevalence and Associated-Factors forIranian journal of public health · 2020Article
- Protozoans in subgingival biofilm: clinical and bacterial associated factors and impact of scaling and root planing treatment.Journal of oral microbiology · 2020Article
- Prevalence of two Entamoeba gingivalis ST1 and ST2-kamaktli subtypes in the human oral cavity under various conditions.Parasitology research · 2018Article
- A new subtype of Entamoeba gingivalis: "E. gingivalis ST2, kamaktli variant".Parasitology research · 2018Article
- Oral microbiome and health.AIMS microbiology · 2018Review
- Reassessing the Role ofFrontiers in cellular and infection microbiology · 2018Review
- The Effect of Nonsurgical Periodontal Therapy on Trichomonas Tenax and Entamoeba Gingivalis in Patients with Chronic Periodontitis.Journal of dentistry (Shiraz, Iran) · 2016Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Investigators using light microscopy have identified the protozoan parasite Entamoeba gingivalis from diseased gingival pockets for nearly 100 years. The objective of the present investigation was to develop a molecular biology approach for determining the presence of E. gingivalis in both diseased gingival pockets and healthy gingival sites. For this, a previously developed conventional polymerase chain reaction (PCR) was evaluated and a real-time polymerase chain reaction assay was developed. Paper points were inserted into the base of the sulcus of both diseased gingival pockets and healthy gingival sites. DNA was extracted using the QIAamp DNA mini kit, and subsequently analyzed using conventional and real-time PCR analysis. A previously described primer set specific for the small subunit ribosomal RNA gene (SSU rDNA) of E. gingivalis was used for the conventional PCR. For the real-time PCR, a primer set was designed to amplify a 135-bp fragment inside the SSU rDNA of E. gingivalis. A conventional PCR assay detected E. gingivalis in 27% of diseased gingival pockets. The real-time PCR using a different primer set detected protozoa in 69% of diseased pocket sites. Thus, the latter technique proved more sensitive for detection of E. gingivalis. No E. gingivalis were detected in any of the healthy gingival pocket sites using either type of PCR assay. Results support a concept that the presence of E. gingivalis is associated only with diseased gingival pocket sites. The newly described methodology may also serve to provide a novel eukaryotic cell marker of disease status in gingival pockets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.