ArticleNature biotechnology2011
Label-free quantification of membrane-ligand interactions using backscattering interferometry.
Article in Nature biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
47 citing papers in PubMed, 88 citations in OpenAlex.
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- Theoretical Basis for Refractive Index Changes Resulting from Solution Phase Molecular Interaction.The journal of physical chemistry. B · 2025Article
- Electric field-enhanced backscatter interferometry detection for capillary electrophoresis.Scientific reports · 2024Article
- Structural mass spectrometry approaches to understand multidrug efflux systems.Essays in biochemistry · 2023Review
- In Situ Analysis of Membrane-Protein Binding Kinetics and Cell-Surface Adhesion Using Plasmonic Scattering Microscopy.Angewandte Chemie (International ed. in English) · 2022Article
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- Kinetic Microscale Thermophoresis for Simultaneous Measurement of Binding Affinity and Kinetics.Angewandte Chemie (International ed. in English) · 2021Article
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- Revisiting peptidoglycan sensing: interactions with host immunity and beyond.Chemical communications (Cambridge, England) · 2020Review
- Unlabeled lysophosphatidic acid receptor binding in free solution as determined by a compensated interferometric reader.Journal of lipid research · 2020Article
- Measuring Stepwise Binding of Thermally Fluctuating Particles to Cell Membranes without Fluorescence.Biophysical journal · 2020Article
- A Novel Agonist of the Type 1 Lysophosphatidic Acid Receptor (LPAJournal of medicinal chemistry · 2020Article
- Quantifying Ligand-Protein Binding Kinetics with Self-Assembled Nano-oscillators.Analytical chemistry · 2019Article
- Lysophospholipid G protein-coupled receptor binding parameters as determined by backscattering interferometry.Journal of lipid research · 2019Article
- Measuring Ligand Binding Kinetics to Membrane Proteins Using Virion Nano-oscillators.Journal of the American Chemical Society · 2018Article
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5 authors at 2 institutions in 1 country.
Funding
Abstract
Although membrane proteins are ubiquitous within all living organisms and represent the majority of drug targets, a general method for direct, label-free measurement of ligand binding to native membranes has not been reported. Here we show that backscattering interferometry (BSI) can accurately quantify ligand-receptor binding affinities in a variety of membrane environments. By detecting minute changes in the refractive index of a solution, BSI allows binding interactions of proteins with their ligands to be measured at picomolar concentrations. Equilibrium binding constants in the micromolar to picomolar range were obtained for small- and large-molecule interactions in both synthetic and cell-derived membranes without the use of labels or supporting substrates. The simple and low-cost hardware, high sensitivity and label-free nature of BSI should make it readily applicable to the study of many membrane-associated proteins of biochemical and pharmacological interest.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.