ArticleNature methods2011
Two-color nanoscopy of three-dimensional volumes by 4Pi detection of stochastically switched fluorophores.
Article in Nature methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 95 papers.
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Who cites it
95 citing papers in PubMed.
- Interferometric ultra-high resolution 3D imaging through brain sections.Nature communications · 2026Article
- Mirror-enhanced 4Pi-SMLM with one objective enables isotropic nanoscale imaging.Nature biotechnology · 2026Article
- Molecular-scale isotropic 3D super-resolution microscopy via interference localization.Nature methods · 2026Article
- 4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination.Nature methods · 2026Article
- Disrupted theta synchronization and synaptic connectivity in the visual cortex of Fmr1 KO mice.Nature communications · 2025Article
- Cellular optical imaging techniques: a dynamic advancing frontier.Science China. Life sciences · 2025Review
- Quantifying the Shape and Stiffness of Single Extracellular Vesicles in Aqueous Solution via Membrane Diffusivity Measurements.Chemical & biomedical imaging · 2025Article
- Elucidating subcellular architecture and dynamics at isotropic 100-nm resolution with 4Pi-SIM.Nature methods · 2025Article
- System- and sample-agnostic isotropic three-dimensional microscopy by weakly physics-informed, domain-shift-resistant axial deblurring.Nature communications · 2025Article
- Single-molecule orientation-localization microscopy: Applications and approaches.Quarterly reviews of biophysics · 2024Review
- Lanthanide ion-doped upconversion nanoparticles for low-energy super-resolution applications.Light, science & applications · 2024Review
- Universal inverse modeling of point spread functions for SMLM localization and microscope characterization.Nature methods · 2024Article
- Advanced Imaging Techniques for the Characterization of Subcellular Organelle Structure in Pancreatic Islet β Cells.Comprehensive Physiology · 2023Article
- Universal inverse modelling of point spread functions for SMLM localization and microscope characterization.bioRxiv : the preprint server for biology · 2023Article
- Force generation in human blood platelets by filamentous actomyosin structures.Biophysical journal · 2023Article
- Recent Advances in Single-Molecule Tracking and Imaging Techniques.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023Review
- Metal-Induced Energy Transfer (MIET) for Live-Cell Imaging with Fluorescent Proteins.ACS nano · 2023Article
- Photoactivatable Fluorophores for Bioimaging Applications.ACS applied optical materials · 2023Article
- Three-dimensional single particle tracking using 4π self-interference of temporally phase-shifted fluorescence.Light, science & applications · 2023Article
- Optimal precision and accuracy in 4Pi-STORM using dynamic spline PSF models.Nature methods · 2022Article
35 more citing papers are in PubMed but not listed here.
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9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We demonstrate three-dimensional (3D) super-resolution imaging of stochastically switched fluorophores distributed across whole cells. By evaluating the higher moments of the diffraction spot provided by a 4Pi detection scheme, single markers can be simultaneously localized with <10 nm precision in three dimensions in a layer of 650 nm thickness at an arbitrarily selected depth in the sample. By splitting the fluorescence light into orthogonal polarization states, our 4Pi setup also facilitates the 3D nanoscopy of multiple fluorophores. Offering a combination of multicolor recording, nanoscale resolution and extended axial depth, our method substantially advances the noninvasive 3D imaging of cells and of other transparent materials.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.