ArticleThe EMBO journal2011
Mg2+ facilitates leader peptide translation to induce riboswitch-mediated transcription termination.
Article in The EMBO journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Global regulation via modulation of ribosome pausing by the ABC-F protein EttA.Nature communications · 2024Article
- A novel regulatory interplay between atypical B12 riboswitches and uORF translation in Mycobacterium tuberculosis.Nucleic acids research · 2024Article
- Regulation of magnesium ion transport inRNA biology · 2024Article
- Nascent polypeptide within the exit tunnel stabilizes the ribosome to counteract risky translation.The EMBO journal · 2021Article
- mRNA dynamics and alternative conformations adopted under low and high arginine concentrations control polyamine biosynthesis in Salmonella.PLoS genetics · 2019Article
- Protein synthesis controls phosphate homeostasis.Genes & development · 2018Article
- Osmosensing by the bacterial PhoQ/PhoP two-component system.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Metalloriboswitches: RNA-based inorganic ion sensors that regulate genes.The Journal of biological chemistry · 2017Review
- Mg2+ regulates transcription of mgtA in Salmonella Typhimurium via translation of proline codons during synthesis of the MgtL peptide.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Reducing Ribosome Biosynthesis Promotes Translation during Low MgMolecular cell · 2016Article
- Unusually long-lived pause required for regulation of a Rho-dependent transcription terminator.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Genetic analysis of riboswitch-mediated transcriptional regulation responding to Mn2+ in Salmonella.The Journal of biological chemistry · 2014Article
- Signal-specific temporal response by the Salmonella PhoP/PhoQ regulatory system.Molecular microbiology · 2014Article
- Tandem attenuators control expression of the Salmonella mgtCBR virulence operon.Molecular microbiology · 2012Article
- Riboswitch control of Rho-dependent transcription termination.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Metabolite recognition principles and molecular mechanisms underlying riboswitch function.Annual review of biophysics · 2012Review
- Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have characterized a 17-residue peptide, MgtL, which is translated specifically in high Mg(2+) from an open reading frame (ORF) embedded in the Mg(2+) riboswitch domain, previously identified in the 5' leader region of Mg(2+) transporter gene mgtA in Salmonella. We demonstrate that mgtL translation is required to prematurely terminate mgtA transcription. Abrogation of mgtL translation by mutation of its start codon results in transcription of the mgtA-coding region in high Mg(2+), suggesting that ribosome stalling is not required for preventing premature transcription termination. Consistently, the Mg(2+) riboswitch responds to cytoplasmic Mg(2+), but not to proline or arginine, both repeatedly present in the MgtL sequence, to mediate mgtL translation-coupled regulation. RNA structural probing and nucleotide substitution analysis show that the riboswitch loop A region alters base pairing in response to Mg(2+), and favours stem-loop A1 in high Mg(2+), subsequently opening the ribosome-binding sequence for mgtL translation. Presumably, mgtL ORF directs translation to localize a ribosome in cis to act on downstream RNA in a manner similar to some upstream ORFs in prokaryotes and eukaryotes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.