ArticleNucleic acids research2011
Escherichia coli σ⁷⁰ senses sequence and conformation of the promoter spacer region.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Core elements play distinct roles in promoter birth and transcriptional regulation.Nature communications · 2026Article
- Review
- Bidirectional promoters in Escherichia coli: regulatory rules and implications for gene expression noise.Nucleic acids research · 2026Article
- Structural insights into σ28-dependent transcription initiation and its regulation by anti-sigma factor in Pseudomonas aeruginosa.Nucleic acids research · 2026Article
- Unraveling the start element and regulatory divergence of core promoters across the domain bacteria.Nucleic acids research · 2025Article
- A single rare σ70 variant establishes a unique gene expression pattern in the E. coli pathobiont LF82.Nucleic acids research · 2024Article
- Non-canonical transcriptional start sites in E. coli O157:H7 EDL933 are regulated and appear in surprisingly high numbers.BMC microbiology · 2023Article
- Development of a Tetracycline-Inducible System for Conditional Gene Expression in Lactococcus lactis and Streptococcus thermophilus.Microbiology spectrum · 2023Article
- An unexpected abundance of bidirectional promoters withinMicrobiology (Reading, England) · 2023Article
- Rhodobacter sphaeroides CarD Negatively Regulates Its Own Promoter.Journal of bacteriology · 2021Article
- Widespread divergent transcription from bacterial and archaeal promoters is a consequence of DNA-sequence symmetry.Nature microbiology · 2021Article
- The Context-Dependent Influence of Promoter Sequence Motifs on Transcription Initiation Kinetics and Regulation.Journal of bacteriology · 2021Review
- Vibrio cholerae FruR facilitates binding of RNA polymerase to the fru promoter in the presence of fructose 1-phosphate.Nucleic acids research · 2021Article
- Extensive Reannotation of the Genome of the Model StreptomyceteFrontiers in microbiology · 2021Article
- The expression of the acarbose biosynthesis gene cluster in Actinoplanes sp. SE50/110 is dependent on the growth phase.BMC genomics · 2020Article
- Article
- A non-canonical promoter element drives spurious transcription of horizontally acquired bacterial genes.Nucleic acids research · 2020Article
- Two paralogous EcfG σ factors hierarchically orchestrate the activation of the General Stress Response in Sphingopyxis granuli TFA.Scientific reports · 2020Article
- Engineering strong and stress-responsive promoters inSynthetic and systems biotechnology · 2019Article
- The Escherichia coli MarA protein regulates the ycgZ-ymgABC operon to inhibit biofilm formation.Molecular microbiology · 2019Article
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4 authors.
Funding
Abstract
In bacteria, promoter identification by RNA polymerase is mediated by a dissociable σ factor. The housekeeping σ(70) factor of Escherichia coli recognizes two well characterized DNA sequence elements, known as the '-10' and '-35' hexamers. These elements are separated by 'spacer' DNA, the sequence of which is generally considered unimportant. Here, we use a combination of bioinformatics, genetics and biochemistry to show that σ(70) can sense the sequence and conformation of the promoter spacer region. Our data illustrate how alterations in spacer region sequence can increase promoter activity. This stimulatory effect requires σ(70) side chain R451, which is located in close proximity to the non-template strand at promoter position -18. Conversely, R451 is not required to mediate transcriptional stimulation by improvement of the -10 element. Mutation of σ(70) residue R451, which is highly conserved, results in reduced growth rate, consistent with a central role in promoter recognition.
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