ArticleClinical chemistry2011
Rapid and sensitive detection of troponin I in human whole blood samples by using silver nanoparticle films and microwave heating.
Article in Clinical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 42 citations in OpenAlex.
- A Plasmonic Nanoledge Array Sensor for Selective Detection of Cardiovascular Disease Biomarkers in Human Whole Blood.ACS applied nano materials · 2024Article
- Microfluidic devices integrated with plasmonic nanostructures for sensitive fluorescent immunoassays.Biomicrofluidics · 2024Article
- Plasmonic Fluorescence Enhancement in Diagnostics for Clinical Tests at Point-of-Care: A Review of Recent Technologies.Advanced materials (Deerfield Beach, Fla.) · 2023Review
- Review: Applications of surface-enhanced fluorescence (SEF) spectroscopy in bio-detection and biosensing.Sensing and Bio-Sensing Research · 2020Review
- Development of a specific troponin I detection system with enhanced immune sensitivity using a single monoclonal antibody.Royal Society open science · 2020Article
- Nanoscale Technologies for Prevention and Treatment of Heart Failure: Challenges and Opportunities.Chemical reviews · 2019Review
- Plasmon-enhanced optical sensors: a review.The Analyst · 2015Review
- Metal-Enhanced Fluorescence from Silver Nanowires with High Aspect Ratio on Glass Slides for Biosensing Applications.The journal of physical chemistry. C, Nanomaterials and interfaces · 2015Article
- Design and Proof-of-Concept Use of a Circular PMMA Platform with 16-Well Sample Capacity for Microwave-Accelerated Bioassays.Nano biomedicine and engineering · 2013Article
- Rapid and Sensitive Detection of Troponin I-T-C Complex from Human Serum using Microwave-Accelerated Metal-Enhanced Fluorescence.Nano biomedicine and engineering · 2011Article
- Plasmon-Enhanced Fluorescence Biosensors: a Review.Plasmonics (Norwell, Mass.)Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundCardiovascular diseases are among the leading causes of mortality in developed countries. It is widely recognized that troponin I (TnI) can be used for the assessment of a myocardial infarction.
methodsWe investigated the use of the microwave-accelerated and metal-enhanced fluorescence (MA-MEF), a technique based on the combined use of low-power microwave heating, silver nanoparticle films (SNFs), and fluorescence spectroscopy for the detection of TnI from human whole blood samples. SNFs were deposited onto amine-modified glass microscope slides by use of Tollen's reaction scheme and characterized by optical absorption spectroscopy and scanning electron microscopy. The detection of TnI from buffer solutions and human whole blood samples on SNFs was carried out by using fluorescence-based immunoassays at room temperature (control immunoassay, 2 h total assay time) or microwave heating (MA-MEF-based immunoassay, 1 min total assay time).
resultsWe found that the lower limits of detection for TnI from buffer solutions in the control immunoassay and MA-MEF-based immunoassay were 0.1 μg/L and 0.005 μg/L, respectively. However, we were unable to detect TnI in whole blood samples in the control immunoassays owing to the coagulation of whole blood within 5 min of the incubation step. The use of the MA-MEF technique allowed detection of TnI from whole blood samples in 1 min with a lower detection limit of 0.05 μg/L.
conclusionsThe MA-MEF-based immunoassay is one of the fastest reported quantitative detection methodos for detection of TnI in human whole blood and has low detection limits similar to those obtained with commercially available immunoassays.
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