ArticleJournal of bacteriology2011
Germination of individual Bacillus subtilis spores with alterations in the GerD and SpoVA proteins, which are important in spore germination.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Recent progress in proteins regulating the germination ofJournal of bacteriology · 2025Review
- Characterization of individual spores of two biological insecticides, Bacillus thuringiensis and Lysinibacillus sphaericus, in response to glutaraldehyde using single-cell optical approaches.Archives of microbiology · 2024Article
- Organization and dynamics of the SpoVAEa protein and its surrounding inner membrane lipids, upon germination of Bacillus subtilis spores.Scientific reports · 2022Article
- Occurrence of genes encoding spore germination inMicrobial genomics · 2022Article
- Molecular mechanism of the smart attack of pathogenic bacteria on nematodes.Microbial biotechnology · 2020Article
- The requirement for co-germinants during Clostridium difficile spore germination is influenced by mutations in yabG and cspA.PLoS pathogens · 2019Article
- Slow leakage of Ca-dipicolinic acid from individual bacillus spores during initiation of spore germination.Journal of bacteriology · 2015Article
- Analysis of the dynamics of a Bacillus subtilis spore germination protein complex during spore germination and outgrowth.Journal of bacteriology · 2015Article
- Monitoring of commitment, blocking, and continuation of nutrient germination of individual Bacillus subtilis spores.Journal of bacteriology · 2014Article
- Function of the SpoVAEa and SpoVAF proteins of Bacillus subtilis spores.Journal of bacteriology · 2014Article
- Analysis of the loss in heat and acid resistance during germination of spores of Bacillus species.Journal of bacteriology · 2014Article
- High salinity alters the germination behavior of Bacillus subtilis spores with nutrient and nonnutrient germinants.Applied and environmental microbiology · 2014Article
- Levels of germination proteins in Bacillus subtilis dormant, superdormant, and germinating spores.PloS one · 2014Article
- Monitoring rates and heterogeneity of high-pressure germination of bacillus spores by phase-contrast microscopy of individual spores.Applied and environmental microbiology · 2014Article
- The Clostridium perfringens germinant receptor protein GerKC is located in the spore inner membrane and is crucial for spore germination.Journal of bacteriology · 2013Article
- Expression level of Bacillus subtilis germinant receptors determines the average rate but not the heterogeneity of spore germination.Journal of bacteriology · 2013Article
- Article
- Effects of the SpoVT regulatory protein on the germination and germination protein levels of spores of Bacillus subtilis.Journal of bacteriology · 2012Article
- Germination protein levels and rates of germination of spores of Bacillus subtilis with overexpressed or deleted genes encoding germination proteins.Journal of bacteriology · 2012Article
- Levels of germination proteins in dormant and superdormant spores of Bacillus subtilis.Journal of bacteriology · 2012Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Release of Ca(2+) with dipicolinic acid (CaDPA) was monitored by Raman spectroscopy and differential interference contrast microscopy during germination of individual spores of Bacillus subtilis strains with alterations in GerD and SpoVA proteins. Notable conclusions about germination after the addition of nutrient were as follows. (i) Following L-alanine addition, wild-type and gerD spores and spores with elevated SpoVA protein levels (↑SpoVA spores) slowly released ∼10% of their CaDPA during a variable (6- to 55-min) period ending at T(lag), the time when faster CaDPA release began. (ii) T(lag) times were lower for ↑SpoVA spores than for wild-type spores and were higher for gerD spores. (iii) The long T(lag) times of gerD spores were partially due to slow commitment to germinate. (iv) The intervals between the commitment to germinate and CaDPA release were similar for wild-type and ↑SpoVA spores but longer for gerD spores. (v) The times for rapid CaDPA release, ΔT(release) = T(release) - T(lag) (with T(release) being the time at which CaDPA release was complete), were similar for wild-type, gerD, and ↑SpoVA spores. (vi) Spores with either one of two point mutations in the spoVA operon (spoVA(1) and spoVA(2) spores) exhibited a more rapid rate of CaDPA release beginning immediately after L-alanine addition leading to ∼65% CaDPA release prior to T(lag). (vii) T(lag) times for spoVA(1) and spoVA(2) spores were longer than for wild-type spores. (viii) The intervals between spoVA(1) and spoVA(2) spores' commitment and CaDPA release were similar to those for wild-type spores, but commitment occurred later. In contrast to germination after the addition of nutrient, T(lag) and ΔT(release) times were relatively similar during dodecylamine germination of spores of the five strains. These findings suggest the following. (i) GerD plays no role in CaDPA release during spore germination. (ii) SpoVA proteins are involved in CaDPA release during germination with nutrients, and probably with dodecylamine. (iii) Spores release significant CaDPA before commitment. (iv) CaDPA release during T(lag) and ΔT(release) may signal subsequent germination events.
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