ArticleApplied and environmental microbiology2011
Evaluation of procedures for the collection, processing, and analysis of biomolecules from low-biomass surfaces.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 65 citations in OpenAlex.
- Environmental contamination with carbapenem resistantFrontiers in cellular and infection microbiology · 2024Article
- Analysis of Microbiomes from Ultra-Low Biomass Surfaces Using Novel Surface Sampling and Nanopore Sequencing.Journal of biomolecular techniques : JBT · 2023Article
- Microbial Detection and Quantification of Low-Biomass Water Samples Using an International Space Station Smart Sample Concentrator.Microorganisms · 2023Article
- Metagenomic Methods for Addressing NASA's Planetary Protection Policy Requirements on Future Missions: A Workshop Report.Astrobiology · 2023Article
- The Development of a 3D Printer-Inspired, Microgravity-Compatible Sample Preparation Device for Future Use Inside the International Space Station.Micromachines · 2023Article
- Microbial Burden Estimation of Food Items, Built Environments, and the International Space Station Using Film Media.Microorganisms · 2022Article
- Genomic Characterization ofJournal of fungi (Basel, Switzerland) · 2022Article
- The Isolation and Characterization of Rare Mycobiome Associated With Spacecraft Assembly Cleanrooms.Frontiers in microbiology · 2022Article
- Characterization of Spacesuit Associated Microbial Communities and Their Implications for NASA Missions.Frontiers in microbiology · 2021Article
- Validation of the International Space Station Smart Sample Concentrator for Microbial Monitoring of Low Biomass Water Samples.Journal of biomolecular techniques : JBT · 2020Article
- Article
- Description of Chloramphenicol ResistantFrontiers in microbiology · 2020Article
- Assessing the Risk of Transfer of Microorganisms at the International Space Station Due to Cargo Delivery by Commercial Resupply Vehicles.Frontiers in microbiology · 2020Article
- Article
- Microscopic Characterization of Biological and Inert Particles Associated with Spacecraft Assembly Cleanroom.Scientific reports · 2019Article
- Microbiomes of China's Space Station During Assembly, Integration, and Test Operations.Microbial ecology · 2019Article
- Characterization of the total and viable bacterial and fungal communities associated with the International Space Station surfaces.Microbiome · 2019Article
- Whole metagenome profiles of particulates collected from the International Space Station.Microbiome · 2017Article
- Article
- Microbial Community and Biochemical Dynamics of Biological Soil Crusts across a Gradient of Surface Coverage in the Central Mojave Desert.Frontiers in microbiology · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To comprehensively assess microbial diversity and abundance via molecular-analysis-based methods, procedures for sample collection, processing, and analysis were evaluated in depth. A model microbial community (MMC) of known composition, representative of a typical low-biomass surface sample, was used to examine the effects of variables in sampling matrices, target cell density/molecule concentration, and cryogenic storage on the overall efficacy of the sampling regimen. The MMC used in this study comprised 11 distinct species of bacterial, archaeal, and fungal lineages associated with either spacecraft or clean-room surfaces. A known cellular density of MMC was deposited onto stainless steel coupons, and after drying, a variety of sampling devices were used to recover cells and biomolecules. The biomolecules and cells/spores recovered from each collection device were assessed by cultivable and microscopic enumeration, and quantitative and species-specific PCR assays. rRNA gene-based quantitative PCR analysis showed that cotton swabs were superior to nylon-flocked swabs for sampling of small surface areas, and for larger surfaces, biological sampling kits significantly outperformed polyester wipes. Species-specific PCR revealed differential recovery of certain species dependent upon the sampling device employed. The results of this study empower current and future molecular-analysis-based microbial sampling and processing methodologies.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.