ArticleDiagnostic microbiology and infectious disease2011
Detection of Mycoplasma pneumoniae, Chlamydia pneumoniae, and Legionella spp. in clinical specimens using a single-tube multiplex real-time PCR assay.
Article in Diagnostic microbiology and infectious disease, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed.
- Research progress on detection technologies for Mycoplasma pneumoniae.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Review
- Development of a Real-Time Polymerase Chain Reaction Assay to DetectDiagnostics (Basel, Switzerland) · 2026Article
- Evaluation ofMicrobiology spectrum · 2026Article
- Post-pandemic surge ofJournal of clinical microbiology · 2026Article
- Review
- Atypical pneumonia (Review).Experimental and therapeutic medicine · 2024Review
- Chlamydophila pneumoniae-associated community-acquired pneumonia in paediatric patients of a tertiary care hospital in Mexico: molecular diagnostic and clinical insights.Scientific reports · 2023Article
- Multiplex Real-time PCR Assay for the Detection of allAnnals of laboratory medicine · 2023Article
- VariableMicroorganisms · 2022Article
- Lefamulin in Patients with Community-Acquired Bacterial Pneumonia Caused by Atypical Respiratory Pathogens: Pooled Results from Two Phase 3 Trials.Antibiotics (Basel, Switzerland) · 2021Article
- A single centre study of viral community-acquired pneumonia in children: No evidence of SARS-CoV-2 from October 2019 to March 2020.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2020Article
- Development of a DNA microarray assay for rapid detection of fifteen bacterial pathogens in pneumonia.BMC microbiology · 2020Article
- Evaluation of Commercial Molecular Diagnostic Methods for Detection and Determination of Macrolide Resistance in Mycoplasma pneumoniae.Journal of clinical microbiology · 2020Article
- Challenges and clinical relevance of molecular detection of Bordetella pertussis in South Africa.BMC infectious diseases · 2019Article
- Outbreak of influenza A in a boarding school in South Africa, 2016.The Pan African medical journal · 2019Article
- Detection ofInfection and drug resistance · 2019Article
- Detection of Influenza C Viruses Among Outpatients and Patients Hospitalized for Severe Acute Respiratory Infection, Minnesota, 2013-2016.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2018Article
- Article
- Contemporary Situation of Community-acquired Pneumonia in China: A Systematic Review.Journal of translational internal medicine · 2018Article
- Epidemiology and Molecular Identification and Characterization of Mycoplasma pneumoniae, South Africa, 2012-2015.Emerging infectious diseases · 2018Article
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5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A multiplex real-time PCR assay for the detection of Mycoplasma pneumoniae (MP181), Chlamydia (Chlamydophila) pneumoniae (CP-Arg), Legionella spp. (Pan-Leg), and the human RNase P (RNase P) gene was developed for rapid testing of atypical bacterial respiratory pathogens in clinical specimens. This method uses 4 distinct hydrolysis probes to detect 3 leading causes of community-acquired pneumonia. The assay was evaluated for specificity and sensitivity by testing against 35 related organisms, a dilution series of each specific target and 197 clinical specimens. Specificity testing demonstrated no cross-reactivity. A comparison to previously validated singleplex real-time PCR assays for each agent was also performed. The analytical sensitivity for specific pathogen targets in both the singleplex and multiplex was identical (50 fg), while efficiencies ranged from 82% to 97% for the singleplex assays and from 90% to 100% for the multiplex assay. The clinical sensitivity of the multiplex assay was improved for the Pan-Leg and CP-Arg targets when compared to the singleplex. The MP181 assay displayed equivalent performance. This multiplex assay provides an overall improvement in the diagnostic capability for these agents by demonstrating a sensitive, high-throughput and rapid method. This procedure may allow for a practical and efficient means to test respiratory clinical specimens for atypical pneumonia agents in health care settings and facilitate an appropriate public health response to outbreaks.
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