ArticleBiomaterials2011
The generation of biomolecular patterns in highly porous collagen-GAG scaffolds using direct photolithography.
Article in Biomaterials, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 85 citations in OpenAlex.
- Relyophilized collagen scaffold to improve handling and small molecule loading.bioRxiv : the preprint server for biology · 2026Article
- Role of Cell Adhesion in Cancer Metastasis Formation: A Review.ACS omega · 2025Review
- Sequential sequestrations increase the incorporation and retention of multiple growth factors in mineralized collagen scaffolds.RSC advances · 2020Article
- Immobilization of Growth Factors for Cell Therapy Manufacturing.Frontiers in bioengineering and biotechnology · 2020Review
- Modification of the Surface Properties of AlACS omega · 2019Article
- A versatile method for the UVA-induced cross-linking of acetophenone- or benzophenone-functionalized DNA.Scientific reports · 2018Article
- Incorporating β-cyclodextrin into collagen scaffolds to sequester growth factors and modulate mesenchymal stem cell activity.Acta biomaterialia · 2018Article
- Visualizing Intrapopulation Hematopoietic Cell Heterogeneity with Self-Organizing Maps of SIMS Data.Tissue engineering. Part C, Methods · 2018Article
- Application of laser scanning confocal microscopy in the soft tissue exquisite structure for 3D scan.International journal of burns and trauma · 2018Review
- Cell Microarray Technologies for High-Throughput Cell-Based Biosensors.Sensors (Basel, Switzerland) · 2017Review
- Synthesis of and in vitro and in vivo evaluation of a novel TGF-β1-SF-CS three-dimensional scaffold for bone tissue engineering.International journal of molecular medicine · 2016Article
- Increasing the strength and bioactivity of collagen scaffolds using customizable arrays of 3D-printed polymer fibers.Acta biomaterialia · 2016Article
- The use of covalently immobilized stem cell factor to selectively affect hematopoietic stem cell activity within a gelatin hydrogel.Biomaterials · 2015Article
- The induction of pro-angiogenic processes within a collagen scaffold via exogenous estradiol and endometrial epithelial cells.Biotechnology and bioengineering · 2015Article
- Collagen Scaffolds Incorporating Coincident Gradations of Instructive Structural and Biochemical Cues for Osteotendinous Junction Engineering.Advanced healthcare materials · 2015Article
- The effect of glycosaminoglycan content on polyethylenimine-based gene delivery within three-dimensional collagen-GAG scaffolds.Biomaterials science · 2015Article
- Collagen scaffold arrays for combinatorial screening of biophysical and biochemical regulators of cell behavior.Advanced healthcare materials · 2015Article
- Capturing relevant extracellular matrices for investigating cell migration.F1000Research · 2015Review
- The combined effects of matrix stiffness and growth factor immobilization on the bioactivity and differentiation capabilities of adipose-derived stem cells.Biomaterials · 2014Article
- Strategies to balance covalent and non-covalent biomolecule attachment within collagen-GAG biomaterials.Biomaterials science · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The extracellular matrix (ECM) is a complex organization of structural proteins found within tissues and organs. Heterogeneous tissues with spatially and temporally modulated properties play an important role in organism physiology. Here we present a benzophenone (BP) based direct, photolithographic approach to spatially pattern solution phase biomolecules within collagen-GAG (CG) scaffolds and demonstrate creation of a wide range of patterns composed of multiple biomolecular species in a manner independent from scaffold fabrication steps. We demonstrate the ability to immobilize biomolecules at surface densities of up to 1000 ligands per square micron on the scaffold strut surface and to depths limited by the penetration depth of the excitation source into the scaffold structure. Importantly, while BP photopatterning does further crosslink the CG scaffold, evidenced by increased mechanical properties and collagen crystallinity, it does not affect scaffold microstructural or compositional properties or negatively influence cell adhesion, viability, or proliferation. We show that covalently photoimmobilized fibronectin within a CG scaffold significantly increases the speed of MC3T3-E1 cell attachment relative to the bare CG scaffold or non-specifically adsorbed fibronectin, suggesting that this approach can be used to improve scaffold bioactivity. Our findings, on the whole, establish the use of direct, BP photolithography as a methodology for covalently incorporating activity-improving biochemical cues within 3D collagen biomaterial scaffolds with spatial control over biomolecular deposition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.