Evidence map›Paper›PMID 21397007›Full record

ArticleFree radical biology & medicine2011

Metabolic alterations in yeast lacking copper-zinc superoxide dismutase.

Sadaf Sehati, Matthew H S Clement, Jake Martins, Lei Xu, Valter D Longo, Joan S Valentine, Edith B Gralla

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Free radical biology & medicine, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.5field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 28 citations in OpenAlex.

  1. Article
  2. Sod1 integrates oxygen availability to redox regulate NADPH production and the thiol redoxome.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  3. Review
  4. Examination of SOD1 aggregation modulators and their effect on SOD1 enzymatic activity as a proxy for potential toxicity.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020
    Article
  5. Article
  6. Article
  7. Review
  8. The Expanding Landscape of Moonlighting Proteins in Yeasts.Microbiology and molecular biology reviews : MMBR · 2016
    Review
  9. Stressing Out Hsp90 in Neurotoxic Proteinopathies.Current topics in medicinal chemistry · 2016
    Review
  10. Review
  11. Article
  12. Review
  13. Review
  14. Article
  15. Article
  16. Article
  17. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Sadaf SehatiDepartment of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA 90095-1569, USA.
Matthew H S Clement
Jake Martins
Lei Xu
Valter D Longo
Joan S Valentine
Edith B Gralla
University of California, Los Angeles · USUniversity of Southern California · US

Funding

CHEMISTRY BIOLOGY INTERFACE TRAINING PROGRAMT32GM008496 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MAYNARD, HEATHER D · 1993 to 2019
$6.3M
FUNCTIONAL STUDIES OF CYTOSOLIC ANTIOXIDANT PROTEINSR01DK046828 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI VALENTINE, JOAN SELVERSTONE · 1993 to 2010
$3.0M
MINORITY PREDOCTORAL FELLOWSHIP PROGRAMF31GM077083 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MARTINS, JAKE III · 2006 to 2008
$90k
NIDDK NIH HHS DK46828NIDDK NIH HHS R01 DK046828NIGMS NIH HHS F31 GM077083NIGMS NIH HHS T32 GM008496
6 · The paper itself

Abstract

Yeast lacking copper-zinc superoxide dismutase (sod1∆) have a number of oxygen-dependent defects, including auxotrophies for lysine and methionine and sensitivity to oxygen. Here we report additional defects in metabolic regulation. Under standard growth conditions with glucose as the carbon source, yeast undergo glucose repression in which mitochondrial respiration is deemphasized, energy is mainly derived from glycolysis, and ethanol is produced. When glucose is depleted, the diauxic shift is activated, in which mitochondrial respiration is reemphasized and stress resistance increases. We find that both of these programs are adversely affected by the lack of Sod1p. Key events in the diauxic shift do not occur and sod1∆ cells do not utilize ethanol and stop growing. The ability to shift to growth on ethanol is gradually lost as time in culture increases. In early stages of culture, sod1∆ cells consume more oxygen and have more mitochondrial mass than wild-type cells, indicating that glucose repression is not fully activated. These changes are at least partially dependent on the activity of the Hap2,3,4,5 complex, as indicated by CYC1-lacZ reporter assays. These changes may indicate a role for superoxide in metabolic signaling and regulation and/or a role for glucose derepression in defense against oxidative stress.

Indexed as

Cell Growth ProcessesCell RespirationCytochromes cEthanolGlucoseLysineMethionineMitochondriaMutationOxidative StressOxygenOxygen ConsumptionSaccharomyces cerevisiae ProteinsSuperoxide DismutaseYeastsCYC1 protein, S cerevisiaeCytochromes cEthanolGlucoseLysineMethionineOxygenSaccharomyces cerevisiae ProteinsSuperoxide Dismutase

Identifiers

PMID21397007
PMCPMC3090513
OpenAlexW2011442117

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.