ArticleDifferentiation; research in biological diversity2011
GROα regulates human embryonic stem cell self-renewal or adoption of a neuronal fate.
Article in Differentiation; research in biological diversity, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- The Importance of CXCL1 in the Physiological State and in Noncancer Diseases of the Oral Cavity and Abdominal Organs.International journal of molecular sciences · 2022Review
- CXCL14 Maintains hESC Self-Renewal through Binding to IGF-1R and Activation of the IGF-1R Pathway.Cells · 2020Article
- CXCL1 and CXCL2 Inhibit the Axon Outgrowth in a Time- and Cell-Type-Dependent Manner in Adult Rat Dorsal Root Ganglia Neurons.Neurochemical research · 2019Article
- Obesity-Induced Cellular Senescence Drives Anxiety and Impairs Neurogenesis.Cell metabolism · 2019Article
- Targeting senescence to delay progression of multiple sclerosis.Journal of molecular medicine (Berlin, Germany) · 2018Review
- Generation and post-injury integration of human spinal cord neural stem cells.Nature methods · 2018Article
- Protein profiling identified key chemokines that regulate the maintenance of human pluripotent stem cells.Scientific reports · 2017Article
- CREB coactivators CRTC2 and CRTC3 modulate bone marrow hematopoiesis.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Creatine Enhances Transdifferentiation of Bone Marrow Stromal Cell-Derived Neural Stem Cell Into GABAergic Neuron-Like Cells Characterized With Differential Gene Expression.Molecular neurobiology · 2017Article
- Senescent cells expose and secrete an oxidized form of membrane-bound vimentin as revealed by a natural polyreactive antibody.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Impact of Feeding Strategies on the Scalable Expansion of Human Pluripotent Stem Cells in Single-Use Stirred Tank Bioreactors.Stem cells translational medicine · 2016Article
- CXCR2 and CXCL4 regulate survival and self-renewal of hematopoietic stem/progenitor cells.Blood · 2016Article
- CXCR2 Inhibition in Human Pluripotent Stem Cells Induces Predominant Differentiation to Mesoderm and Endoderm Through Repression of mTOR, β-Catenin, and hTERT Activities.Stem cells and development · 2016Article
- CXCR2 and its related ligands play a novel role in supporting the pluripotency and proliferation of human pluripotent stem cells.Stem cells and development · 2015Article
- The role of senescent cells in ageing.Nature · 2014Review
- Cell senescence: role in aging and age-related diseases.Interdisciplinary topics in gerontology · 2014Review
- Mitochondrial DNA damage induces apoptosis in senescent cells.Cell death & disease · 2013Article
- Sprouty genes regulate proliferation and survival of human embryonic stem cells.Scientific reports · 2013Article
- Aging, cellular senescence, and cancer.Annual review of physiology · 2013Review
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Previously we reported that feeders formed from human placental fibroblasts (hPFs) support derivation and long-term self-renewal of human embryonic stem cells (hESCs) under serum-free conditions. Here, we show, using antibody array and ELISA platforms, that hPFs secrete ∼6-fold higher amounts of the CXC-type chemokine, GROα, than IMR 90, a human lung fibroblast line, which does not support hESC growth. Furthermore, immunocytochemistry and immunoblot approaches revealed that hESCs express CXCR, a GROα receptor. We used this information to develop defined culture medium for feeder-free propagation of hESCs in an undifferentiated state. Cells passaged as small aggregates and maintained in the GROα-containing medium had a normal karyotype, expressed pluripotency markers, and exhibited apical-basal polarity, i.e., had the defining features of pluripotent hESCs. They also differentiated into the three primary (embryonic) germ layers and formed teratomas in immunocompromised mice. hESCs cultured as single cells in the GROα-containing medium also had a normal karyotype, but they downregulated markers of pluripotency, lost apical-basal polarity, and expressed markers that are indicative of the early stages of neuronal differentiation-βIII tubulin, vimentin, radial glial protein, and nestin. These data support our hypothesis that establishing and maintaining cell polarity is essential for the long-term propagation of hESCs in an undifferentiated state and that disruption of cell-cell contacts can trigger adoption of a neuronal fate.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.