ArticlePlant methods2011
Development of expression vectors based on pepino mosaic virus.
Article in Plant methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 70 citations in OpenAlex.
- Specific and efficient translation inhibition-transcription activation resistance module against viruses in plants.Science advances · 2026Article
- Viral vector-based imaging uncovers infection-induced re-localization of pepino mosaic virus proteins.Journal of virology · 2026Article
- Plant Viral Vectors for Vaccine Development.Vaccines · 2026Review
- Pepino Mosaic Virus: A Globally Important Tomato Pathogen and a Rising Model in Molecular Virology.Molecular plant pathology · 2026Review
- Development of Stable Infectious cDNA Clones of Tomato Black Ring Virus Tagged with Green Fluorescent Protein.Viruses · 2024Article
- The tomato calcium-permeable channel 4.1 (SlOSCA4.1) is a susceptibility factor for pepino mosaic virus.Plant biotechnology journal · 2023Article
- A regulatory role for the redox status of the pepino mosaic virus coat protein.PLoS pathogens · 2023Article
- Foxtail mosaic virus: A tool for gene function analysis in maize and other monocots.Molecular plant pathology · 2023Review
- Fluorescently labelled pepino mosaic virus strains share infected tissues and colocalize in cellular aggregates reminiscent of viral replication complexes.Virus research · 2023Article
- Transcriptome analyses unveiled differential regulation of AGO and DCL genes by pepino mosaic virus strains.Molecular plant pathology · 2022Article
- Role of an RNA pseudoknot involving the polyA tail in replication of Pepino mosaic potexvirus and related plant viruses.Scientific reports · 2022Article
- Article
- A cassava common mosaic virus vector for virus-induced gene silencing in cassava.Plant methods · 2021Article
- Article
- Review
- Assessment of the Current Status of Potyviruses in Watermelon and Pumpkin Crops in Spain: Epidemiological Impact of Cultivated Plants and Mixed Infections.Plants (Basel, Switzerland) · 2021Article
- Development and optimization of a pepino mosaic virus-based vector for rapid expression of heterologous proteins in plants.Applied microbiology and biotechnology · 2021Article
- Increasing temperature alters the within-host competition of viral strains and influences virus genetic variability.Virus evolution · 2021Article
- Determinants of Persistent Patterns of Pepino Mosaic Virus Mixed Infections.Frontiers in microbiology · 2021Article
- A cucumber green mottle mosaic virus vector for virus-induced gene silencing in cucurbit plants.Plant methods · 2020Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPlant viruses are useful expression vectors because they can mount systemic infections allowing large amounts of recombinant protein to be produced rapidly in differentiated plant tissues. Pepino mosaic virus (PepMV) (genus Potexvirus, family Flexiviridae), a widespread plant virus, is a promising candidate expression vector for plants because of its high level of accumulation in its hosts and the absence of severe infection symptoms. We report here the construction of a stable and efficient expression vector for plants based on PepMV.
resultsAgroinfectious clones were produced from two different PepMV genotypes (European and Chilean), and these were able to initiate typical PepMV infections. We explored several strategies for vector development including coat protein (CP) replacement, duplication of the CP subgenomic promoter (SGP) and the creation of a fusion protein using the foot-and-mouth disease virus (FMDV) 2A catalytic peptide. We found that CP replacement vectors were unable to move systemically and that vectors with duplicated SGPs (even heterologous SGPs) suffered from significant transgene instability. The fusion protein incorporating the FMDV 2A catalytic peptide gave by far the best results, maintaining stability through serial passages and allowing the accumulation of GFP to 0.2-0.4 g per kg of leaf tissue. The possible use of PepMV as a virus-induced gene silencing vector to study gene function was also demonstrated. Protocols for the use of this vector are described.
conclusionsA stable PepMV vector was generated by expressing the transgene as a CP fusion using the sequence encoding the foot-and-mouth disease virus (FMDV) 2A catalytic peptide to separate them. We have generated a novel tool for the expression of recombinant proteins in plants and for the functional analysis of virus and plant genes. Our experiments have also highlighted virus requirements for replication in single cells as well as intercellular and long-distance movement.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.