ArticleTissue engineering. Part A2011
A multicellular approach forms a significant amount of tissue-engineered small intestine in the mouse.
Article in Tissue engineering. Part A, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 90 citations in OpenAlex.
- Biological Insights into Intestinal Adaptation from Preclinical Models of Short Bowel Syndrome.Cells · 2026Review
- Development of a novel tubular scaffold for tissue-engineered small intestine.Journal of pediatric surgery · 2026Article
- Current trends and research topics regarding organoids: A bibliometric analysis of global research from 2000 to 2023.Heliyon · 2024Article
- Engineered Synthetic Matrices for Human Intestinal Organoid Culture and Therapeutic Delivery.Advanced materials (Deerfield Beach, Fla.) · 2024Review
- In Vivo Transplantation of Human Intestinal Organoids Enhances Select Tight Junction Gene Expression.The Journal of surgical research · 2021Article
- TNFα regulates intestinal organoids from mice with both defined and conventional microbiota.International journal of biological macromolecules · 2020Article
- R-Spondin 1 (RSPO1) Increases Mouse Intestinal Organoid Unit Size and SurvivalFrontiers in bioengineering and biotechnology · 2020Article
- Production of Tissue-Engineered Small Intestine in Rats with Different Ages of Cell Donors.Tissue engineering. Part A · 2019Article
- Intestinal Crypt Organoid: Isolation of Intestinal Stem Cells, In Vitro Culture, and Optical Observation.Methods in molecular biology (Clifton, N.J.) · 2019Article
- The Development of Newborn Porcine Models for Evaluation of Tissue-Engineered Small Intestine.Tissue engineering. Part C, Methods · 2018Article
- Critical intestinal cells originate from the host in enteroid-derived tissue-engineered intestine.The Journal of surgical research · 2018Article
- Composite Scaffolds Based on Intestinal Extracellular Matrices and Oxidized Polyvinyl Alcohol: A Preliminary Study for a New Regenerative Approach in Short Bowel Syndrome.BioMed research international · 2018Article
- Advancing Intestinal Organoid Technology Toward Regenerative Medicine.Cellular and molecular gastroenterology and hepatology · 2018Review
- Tissue Engineering Functional Gastrointestinal Regions: The Importance of Stem and Progenitor Cells.Cold Spring Harbor perspectives in medicine · 2017Review
- Functional Human and Murine Tissue-Engineered Liver Is Generated from Adult Stem/Progenitor Cells.Stem cells translational medicine · 2017Article
- Generating tissue-engineered intestinal epithelium from cultured Lgr5 stem cellsRegenerative therapy · 2016Article
- Establishing Proximal and Distal Regional Identities in Murine and Human Tissue-Engineered Lung and Trachea.Tissue engineering. Part C, Methods · 2016Article
- Production of tissue-engineered intestine from expanded enteroids.The Journal of surgical research · 2016Article
- New approaches to increase intestinal length: Methods used for intestinal regeneration and bioengineering.World journal of transplantation · 2016Review
- Human and Murine Tissue-Engineered Colon Exhibit Diverse Neuronal Subtypes and Can Be Populated by Enteric Nervous System Progenitor Cells When Donor Colon Is Aganglionic.Tissue engineering. Part A · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tissue-engineered small intestine (TESI) has successfully been used to rescue Lewis rats after massive small bowel resection. In this study, we transitioned the technique to a mouse model, allowing investigation of the processes involved during TESI formation through the transgenic tools available in this species. This is a necessary step toward applying the technique to human therapy. Multicellular organoid units were derived from small intestines of transgenic mice and transplanted within the abdomen on biodegradable polymers. Immunofluorescence staining was used to characterize the cellular processes during TESI formation. We demonstrate the preservation of Lgr5- and DcamKl1-positive cells, two putative intestinal stem cell populations, in proximity to their niche mesenchymal cells, the intestinal subepithelial myofibroblasts (ISEMFs), at the time of implantation. Maintenance of the relationship between ISEMF and crypt epithelium is observed during the growth of TESI. The engineered small intestine has an epithelium containing a differentiated epithelium next to an innervated muscularis. Lineage tracing demonstrates that all the essential components, including epithelium, muscularis, nerves, and some of the blood vessels, are of donor origin. This multicellular approach provides the necessary cell population to regenerate large amounts of intestinal tissue that could be used to treat short bowel syndrome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.