ArticleJournal of the American Chemical Society2011
Single-molecule resolution of interfacial fibrinogen behavior: effects of oligomer populations and surface chemistry.
Article in Journal of the American Chemical Society, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 72 citations in OpenAlex.
- Single Molecule Kinetic Fingerprinting of Glycans on IgA1 Antibodies.Analytical chemistry · 2025Article
- Cell- and Serum-Derived Proteins Act as DAMPs to Activate RAW 264.7 Macrophage-like Cells on Silicone Implants.ACS biomaterials science & engineering · 2024Article
- Single-molecule analysis of DNA-binding proteins from nuclear extracts (SMADNE).Nucleic acids research · 2023Article
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- Linking the foreign body response and protein adsorption to PEG-based hydrogels using proteomics.Biomaterials · 2015Article
- Mechanisms of surface-mediated DNA hybridization.ACS nano · 2014Article
- A bottom-up approach to understanding protein layer formation at solid-liquid interfaces.Advances in colloid and interface science · 2014Review
- Interfacial protein-protein associations.Biomacromolecules · 2014Article
- Single-molecule resolution of protein structure and interfacial dynamics on biomaterial surfaces.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- DNA hairpin stabilization on a hydrophobic surface.Small (Weinheim an der Bergstrasse, Germany) · 2013Article
- Effects of molecular size and surface hydrophobicity on oligonucleotide interfacial dynamics.Biomacromolecules · 2012Article
- Identifying mechanisms of interfacial dynamics using single-molecule tracking.Langmuir : the ACS journal of surfaces and colloids · 2012Review
- Distinguishing positional uncertainty from true mobility in single-molecule trajectories that exhibit multiple diffusive modes.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2012Article
- Apparent activation energies associated with protein dynamics on hydrophobic and hydrophilic surfaces.Biophysical journal · 2012Article
- Connecting rare DNA conformations and surface dynamics using single-molecule resonance energy transfer.ACS nano · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Through the use of single-molecule total internal reflection fluorescence microscopy, the dynamic behavior of fibrinogen was observed at the interface between aqueous solution and various solid surfaces. Multiple populations of objects were observed, as characterized by surface residence times, interfacial diffusion, and fluorescence intensity. On all surfaces, populations exhibited direct links between surface residence time, rate of diffusion, and fluorescence intensity. In particular, longer-lived populations diffused more slowly and exhibited greater fluorescence intensity, leading to the conclusion that the objects represented fibrinogen monomers and discrete oligomer populations (dimers, trimers, etc.), and that these oligomer populations play an important role in the protein-surface interaction because of their long surface residence times. Two or three diffusive modes were observed for most populations, indicating that protein aggregates have multiple mechanisms for interaction with solid substrates. In addition, the fastest diffusive mode is believed to represent a hopping mode that often precedes desorption events. Surprisingly, a monolayer of 5000 Da poly(ethylene glycol) (PEG5000) increased surface residence time and slowed diffusion of fibrinogen relative to bare fused silica or hydrophobically modified fused silica, suggesting that the mechanism of PEG resistance to protein adhesion is more sophisticated than the simple repulsion of individual proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.