ArticleJournal of the American Chemical Society2011
Ultrasensitive DNA microarray biosensing via in situ RNA transcription-based amplification and nanoparticle-enhanced SPR imaging.
Article in Journal of the American Chemical Society, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- A High-Sensitivity Method Based on Advanced Optical Waveguide Technology to Detect HBsAg.Journal of healthcare engineering · 2021Article
- Recent Advances in Surface Plasmon Resonance Imaging Sensors.Sensors (Basel, Switzerland) · 2019Review
- Surface Plasmon Enhanced Light Scattering Biosensing: Size Dependence on the Gold Nanoparticle Tag.Sensors (Basel, Switzerland) · 2019Article
- Plasmonic molecular assays: Recent advances and applications for mobile health.Nano research · 2018Review
- SPRi-Based Biosensing Platforms for Detection of Specific DNA Sequences Using Thiolate and Dithiocarbamate Assemblies.Frontiers in chemistry · 2018Article
- Kinetic and high-throughput profiling of epigenetic interactions by 3D-carbene chip-based surface plasmon resonance imaging technology.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Nanotechnology-Based Surface Plasmon Resonance Affinity Biosensors for In Vitro Diagnostics.International journal of analytical chemistry · 2016Review
- Surface Enzyme Chemistries for Ultrasensitive Microarray Biosensing with SPR Imaging.Langmuir : the ACS journal of surfaces and colloids · 2015Article
- Suspension arrays based on nanoparticle-encoded microspheres for high-throughput multiplexed detection.Chemical Society reviews · 2015Review
- Carbon Substrates: A Stable Foundation for Biomolecular Arrays.Annual review of analytical chemistry (Palo Alto, Calif.) · 2015Review
- Melting dynamics of short dsDNA chains in saline solutions.SpringerPlus · 2015Article
- Evaluation of optical detection platforms for multiplexed detection of proteins and the need for point-of-care biosensors for clinical use.Sensors (Basel, Switzerland) · 2014Review
- Fabrication of DNA microarrays on polydopamine-modified gold thin films for SPR imaging measurements.Langmuir : the ACS journal of surfaces and colloids · 2013Article
- DNAzyme footprinting: detecting protein-aptamer complexation on surfaces by blocking DNAzyme cleavage activity.Journal of the American Chemical Society · 2013Article
- Zeptomole detection of DNA nanoparticles by single-molecule fluorescence with magnetic field-directed localization.Analytical biochemistry · 2012Article
- Tunable biomolecular interaction and fluorescence quenching ability of graphene oxide: application to "turn-on" DNA sensing in biological media.Small (Weinheim an der Bergstrasse, Germany) · 2012Article
- On-chip synthesis of protein microarrays from DNA microarrays via coupled in vitro transcription and translation for surface plasmon resonance imaging biosensor applications.Journal of the American Chemical Society · 2012Article
- On-chip synthesis of RNA aptamer microarrays for multiplexed protein biosensing with SPR imaging measurements.Langmuir : the ACS journal of surfaces and colloids · 2012Article
- Article
- Rapid microarray detection of DNA and proteins in microliter volumes with surface plasmon resonance imaging measurements.Langmuir : the ACS journal of surfaces and colloids · 2011Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
DNA microarrays are invaluable tools for the detection and identification of nucleic acids in biosensing applications. The sensitivity and selectivity of multiplexed single-stranded DNA (ssDNA) surface bioaffinity sensing can be greatly enhanced when coupled to a surface enzymatic reaction. Herein we describe a novel method where the specific sequence-dependent adsorption of a target ssDNA template molecule onto an ssDNA-modified gold microarray is followed with the generation of multiple copies of ssRNA via in situ surface transcription by RNA polymerase. The RNA created on this "generator" element is then detected by specific adsorption onto a second adjacent "detector" element of ssDNA that is complementary to one end of the ssRNA transcript. SPR imaging is then used to detect the subsequent hybridization of cDNA-coated gold nanoparticles with the surface-bound RNA. This RNA transcription-based, dual element amplification method is used to detect ssDNA down to a concentration of 1 fM in a volume of 25 μL (25 zeptomoles).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.