ArticleCytoskeleton (Hoboken, N.J.)2011
Subunit interactions within the Chlamydomonas flagellar spokehead.
Article in Cytoskeleton (Hoboken, N.J.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- The role of junctophilin proteins in cellular function.Physiological reviews · 2022Review
- Structural insights into the cause of humanMolecular biology of the cell · 2021Article
- Distinct architecture and composition of mouse axonemal radial spoke head revealed by cryo-EM.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Structure of the radial spoke head and insights into its role in mechanoregulation of ciliary beating.Nature structural & molecular biology · 2021Article
- Loss of Rsph9 causes neonatal hydrocephalus with abnormal development of motile cilia in mice.Scientific reports · 2020Article
- Rsph4a is essential for the triplet radial spoke head assembly of the mouse motile cilia.PLoS genetics · 2020Article
- Rare Human Diseases: Model Organisms in Deciphering the Molecular Basis of Primary Ciliary Dyskinesia.Cells · 2019Review
- The roles of a flagellar HSP40 ensuring rhythmic beating.Molecular biology of the cell · 2019Article
- RSPH6A is required for sperm flagellum formation and male fertility in mice.Journal of cell science · 2018Article
- General and specific promotion of flagellar assembly by a flagellar nucleoside diphosphate kinase.Molecular biology of the cell · 2017Article
- Radial Spokes-A Snapshot of the Motility Regulation, Assembly, and Evolution of Cilia and Flagella.Cold Spring Harbor perspectives in biology · 2017Review
- Immunofluorescence Analysis and Diagnosis of Primary Ciliary Dyskinesia with Radial Spoke Defects.American journal of respiratory cell and molecular biology · 2015Article
- Article
- Targeted NGS gene panel identifies mutations in RSPH1 causing primary ciliary dyskinesia and a common mechanism for ciliary central pair agenesis due to radial spoke defects.Human molecular genetics · 2014Article
- The Chlamydomonas mutant pf27 reveals novel features of ciliary radial spoke assembly.Cytoskeleton (Hoboken, N.J.) · 2013Article
- Loss-of-function mutations in RSPH1 cause primary ciliary dyskinesia with central-complex and radial-spoke defects.American journal of human genetics · 2013Article
- Protein-protein interactions between intermediate chains and the docking complex of Chlamydomonas flagellar outer arm dynein.FEBS letters · 2013Article
- The MIA complex is a conserved and novel dynein regulator essential for normal ciliary motility.The Journal of cell biology · 2013Article
- A flagellar A-kinase anchoring protein with two amphipathic helices forms a structural scaffold in the radial spoke complex.The Journal of cell biology · 2012Article
- The DPY-30 domain and its flanking sequence mediate the assembly and modulation of flagellar radial spoke complexes.Molecular and cellular biology · 2012Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The radial spoke (RS)/central pair (CP) system in cilia and flagella plays an essential role in the regulation of force generation by dynein, the motor protein that drives cilia/flagella movements. Mechanical and mechanochemicl interactions between the CP and the distal part of the RS, the spokehead, should be crucial for this control; however, the details of interaction are totally unknown. As an initial step toward an understanding of the RS-CP interaction, we examined the protein-protein interactions between the five spokehead proteins (radial spoke protein (RSP)1, RSP4, RSP6, RSP9, and RSP10) and three spoke stalk proteins (RSP2, RSP5, and RSP23), all expressed as recombinant proteins. Three of them were shown to have physiological activities by electroporation-mediated protein delivery into mutants deficient in the respective proteins. Glutathione S-transferase pulldown assays in vitro detected interactions in 10 out of 64 pairs of recombinants. In addition, chemical crosslinking of axonemes using five reagents detected seven kinds of interactions between the RS subunits in situ. Finally, in the mixture of the recombinant spokehead subunits, RSP1, RSP4, RSP6, and RSP9 formed a 7-10S complex as detected by sucrose density gradient centrifugation. It may represent a partial assembly of the spokehead. From these results, we propose a model of interactions taking place between the spokehead subunits.
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