SynthesisPloS one2011
Identification of reference genes across physiological states for qRT-PCR through microarray meta-analysis.
Synthesis in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 2 syntheses or guidelines pooled it, 51 citations in OpenAlex.
- Selection of reference genes for quantitative PCR: identifying reference genes for airway epithelial cells exposed toAmerican journal of physiology. Lung cellular and molecular physiology · 2020Pooled it
- Identification of human housekeeping genes and tissue-selective genes by microarray meta-analysis.PloS one · 2011Pooled it
- MiRNA sequencing of platelet and exosome revealed platelet miR-199b-3p as a potential biomarker in lung adenocarcinoma.Frontiers in immunology · 2025Article
- Targeted DNA Methylation Editing Using an All-in-One System Establishes Paradoxical Activation ofCancers · 2024Article
- Identification of stable reference genes for relative quantification of long RNA expression in urinary extracellular vesicles.Journal of extracellular biology · 2024Article
- Identification of Optimal Reference Genes for qRT-PCR Normalization for Physical Activity Intervention and Omega-3 Fatty Acids Supplementation in Humans.International journal of molecular sciences · 2023Article
- Selection and Validation of Reference Genes for Pan-Cancer in Platelets Based on RNA-Sequence Data.Frontiers in genetics · 2022Article
- Identification of new reference genes with stable expression patterns for gene expression studies using human cancer and normal cell lines.Scientific reports · 2021Article
- Molecular phylogenetic and in silico analysis of glyceraldeyde-3-phosphate dehydrogenase (GAPDH) gene from northern bobwhite quail (Colinus virginianus).Molecular biology reports · 2021Article
- Article
- Constitutive expression of a fluorescent protein reports the size of live human cells.Molecular biology of the cell · 2019Article
- Mechanical and Biochemical Effects of Progesterone on Engineered Cervical Tissue.Tissue engineering. Part A · 2018Article
- Selection of internal references for qRT-PCR assays of human hepatocellular carcinoma cell lines.Bioscience reports · 2017Article
- Systematic identification of human housekeeping genes possibly useful as references in gene expression studies.Molecular medicine reports · 2017Article
- Automated identification of reference genes based on RNA-seq data.Biomedical engineering online · 2017Article
- Systematic Selection of Reference Genes for the Normalization of Circulating RNA Transcripts in Pregnant Women Based on RNA-Seq Data.International journal of molecular sciences · 2017Article
- Identification of reference genes and miRNAs for qRT-PCR in human esophageal squamous cell carcinoma.Medical oncology (Northwood, London, England) · 2017Article
- Identification of reference genes for qPCR analysis during hASC long culture maintenance.PloS one · 2017Article
- Easy-to-use strategy for reference gene selection in quantitative real-time PCR experiments.Naunyn-Schmiedeberg's archives of pharmacology · 2016Article
- Genome-wide identification and characterization of reference genes with different transcript abundances for Streptomyces coelicolor.Scientific reports · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe accuracy of quantitative real-time PCR (qRT-PCR) is highly dependent on reliable reference gene(s). Some housekeeping genes which are commonly used for normalization are widely recognized as inappropriate in many experimental conditions. This study aimed to identify reference genes for clinical studies through microarray meta-analysis of human clinical samples. METHODOLOGY/PRINCIPAL
findingsAfter uniform data preprocessing and data quality control, 4,804 Affymetrix HU-133A arrays performed by clinical samples were classified into four physiological states with 13 organ/tissue types. We identified a list of reference genes for each organ/tissue types which exhibited stable expression across physiological states. Furthermore, 102 genes identified as reference gene candidates in multiple organ/tissue types were selected for further analysis. These genes have been frequently identified as housekeeping genes in previous studies, and approximately 71% of them fall into Gene Expression (GO:0010467) category in Gene Ontology. CONCLUSIONS/SIGNIFICANCE: Based on microarray meta-analysis of human clinical sample arrays, we identified sets of reference gene candidates for various organ/tissue types and then examined the functions of these genes. Additionally, we found that many of the reference genes are functionally related to transcription, RNA processing and translation. According to our results, researchers could select single or multiple reference gene(s) for normalization of qRT-PCR in clinical studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.