ArticlePloS one2011
A survey of new temperature-sensitive, embryonic-lethal mutations in C. elegans: 24 alleles of thirteen genes.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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44 citing papers in PubMed, 70 citations in OpenAlex.
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- C. elegans XMAP215/ZYG-9 and TACC/TAC-1 act at multiple times during oocyte meiotic spindle assembly and promote both spindle pole coalescence and stability.PLoS genetics · 2023Article
- Expression Patterns and Levels of All Tubulin Isotypes Analyzed in GFP Knock-In C. elegans Strains.Cell structure and function · 2021Article
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- Centriole-less pericentriolar material serves as a microtubule organizing center at the base of C. elegans sensory cilia.Current biology : CB · 2021Article
- Interactions between the WEE-1.3 kinase and the PAM-1 aminopeptidase in oocyte maturation and the early C. elegans embryo.G3 (Bethesda, Md.) · 2021Article
- A genetic screen for temperature-sensitive morphogenesis-defective Caenorhabditis elegans mutants.G3 (Bethesda, Md.) · 2021Article
- The molecular underpinnings of fertility: Genetic approaches inAdvanced genetics (Hoboken, N.J.) · 2021Article
- Uncovering the genetic blueprint of theProceedings of the National Academy of Sciences of the United States of America · 2020Article
- The conserved AAA-ATPase PCH-2Molecular biology of the cell · 2020Article
- A portable, low-cost device for precise control of specimen temperature under stereomicroscopes.PloS one · 2020Article
- A behavior-based drug screening system using a Caenorhabditis elegans model of motor neuron disease.Scientific reports · 2019Article
- Kinetochore Recruitment of the Spindle and Kinetochore-Associated (Ska) Complex Is Regulated by Centrosomal PP2A inGenetics · 2019Article
- PP2A-B55/SUR-6 collaborates with the nuclear lamina for centrosome separation during mitotic entry.Molecular biology of the cell · 2019Article
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- Mitotic Cell Division inGenetics · 2019Review
- Spindle assembly checkpoint strength is linked to cell fate in the Caenorhabditis elegans embryo.Molecular biology of the cell · 2018Article
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Authors and funding
20 authors at 1 institution in 1 country.
Funding
Abstract
To study essential maternal gene requirements in the early C. elegans embryo, we have screened for temperature-sensitive, embryonic lethal mutations in an effort to bypass essential zygotic requirements for such genes during larval and adult germline development. With conditional alleles, multiple essential requirements can be examined by shifting at different times from the permissive temperature of 15°C to the restrictive temperature of 26°C. Here we describe 24 conditional mutations that affect 13 different loci and report the identity of the gene mutations responsible for the conditional lethality in 22 of the mutants. All but four are mis-sense mutations, with two mutations affecting splice sites, another creating an in-frame deletion, and one creating a premature stop codon. Almost all of the mis-sense mutations affect residues conserved in orthologs, and thus may be useful for engineering conditional mutations in other organisms. We find that 62% of the mutants display additional phenotypes when shifted to the restrictive temperature as L1 larvae, in addition to causing embryonic lethality after L4 upshifts. Remarkably, we also found that 13 out of the 24 mutations appear to be fast-acting, making them particularly useful for careful dissection of multiple essential requirements. Our findings highlight the value of C. elegans for identifying useful temperature-sensitive mutations in essential genes, and provide new insights into the requirements for some of the affected loci.
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