ArticlePloS one2011
Real-time CARS imaging reveals a calpain-dependent pathway for paranodal myelin retraction during high-frequency stimulation.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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Who cites it
27 citing papers in PubMed, 58 citations in OpenAlex.
- Dynamics of mature myelin.Nature neuroscience · 2024Review
- Vibrational spectroscopy and multiphoton microscopy for label-free visualization of nervous system degeneration and regeneration.Biophysical reviews · 2024Review
- Oligodendrocyte calcium signaling promotes actin-dependent myelin sheath extension.Nature communications · 2024Article
- Practices, Potential, and Perspectives for Detecting Predisease Using Raman Spectroscopy.International journal of molecular sciences · 2023Review
- Distinct Changes in Calpain and Calpastatin during PNS Myelination and Demyelination in Rodent Models.International journal of molecular sciences · 2022Article
- Excitable Axonal Domains Adapt to Sensory Deprivation in the Olfactory System.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Oligodendrocytic NaNature communications · 2021Article
- Completion of neuronal remodeling prompts myelination along developing motor axon branches.The Journal of cell biology · 2021Article
- Neuroinflammation in the normal-appearing white matter (NAWM) of the multiple sclerosis brain causes abnormalities at the nodes of Ranvier.PLoS biology · 2020Article
- Differential effect on myelination through abolition of activity-dependent synaptic vesicle release or reduction of overall electrical activity of selected cortical projections in the mouse.Journal of anatomy · 2019Article
- Nodal Dynamics afterThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Parallel Evaluation of Two Potassium Channel Blockers in Restoring Conduction in Mechanical Spinal Cord Injury in Rat.Journal of neurotrauma · 2018Article
- Polyethylene glycol in spinal cord injury repair: a critical review.Journal of experimental pharmacology · 2018Review
- Article
- Advances in Intravital Non-Linear Optical Imaging of the Central Nervous System in Rodents.Brain plasticity (Amsterdam, Netherlands) · 2016Review
- Imaging MyelinationBrain plasticity (Amsterdam, Netherlands) · 2016Review
- A Novel Approach for Studying the Physiology and Pathophysiology of Myelinated and Non-Myelinated Axons in the CNS White Matter.PloS one · 2016Article
- Assessment of white matter loss using bond-selective photoacoustic imaging in a rat model of contusive spinal cord injury.Journal of neurotrauma · 2014Article
- Primary paranode demyelination modulates slowly developing axonal depolarization in a model of axonal injury.Journal of computational neuroscience · 2014Article
- Label-free real-time imaging of myelination in the Xenopus laevis tadpole by in vivo stimulated Raman scattering microscopy.Journal of biomedical optics · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
High-frequency electrical stimulation is becoming a promising therapy for neurological disorders, however the response of the central nervous system to stimulation remains poorly understood. The current work investigates the response of myelin to electrical stimulation by laser-scanning coherent anti-Stokes Raman scattering (CARS) imaging of myelin in live spinal tissues in real time. Paranodal myelin retraction at the nodes of Ranvier was observed during 200 Hz electrical stimulation. Retraction was seen to begin minutes after the onset of stimulation and continue for up to 10 min after stimulation was ceased, but was found to reverse after a 2 h recovery period. The myelin retraction resulted in exposure of Kv 1.2 potassium channels visualized by immunofluorescence. Accordingly, treating the stimulated tissue with a potassium channel blocker, 4-aminopyridine, led to the appearance of a shoulder peak in the compound action potential curve. Label-free CARS imaging of myelin coupled with multiphoton fluorescence imaging of immuno-labeled proteins at the nodes of Ranvier revealed that high-frequency stimulation induced paranodal myelin retraction via pathologic calcium influx into axons, calpain activation, and cytoskeleton degradation through spectrin break-down.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.