ArticlePloS one2011
Dose-dependent thresholds of 10-ns electric pulse induced plasma membrane disruption and cytotoxicity in multiple cell lines.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 79 citations in OpenAlex.
- Impact of pulse duration on cardiac electroporation: nanosecond pulses enhance cardiomyocyte selectivity and promote a Raman-detected shift towards apoptotic cell death.Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology · 2025Article
- Pulsed Field Ablation for the Treatment of Atrial Fibrillation: A Review and a Look into its Future.The heart surgery forum · 2024Article
- Identification of Proteins Involved in Cell Membrane Permeabilization by Nanosecond Electric Pulses (nsEP).International journal of molecular sciences · 2023Article
- Rapid and precise tracking of water influx and efflux across cell membranes induced by a pulsed electric field.Biomedical optics express · 2023Article
- Pulsed Electric Field Ablation of Esophageal Malignancies and Mitigating Damage to Smooth Muscle: An In Vitro Study.International journal of molecular sciences · 2023Article
- Pulse width and intensity effects of pulsed electric fields on cancerous and normal skin cells.Scientific reports · 2022Article
- Electroporation and cell killing by milli- to nanosecond pulses and avoiding neuromuscular stimulation in cancer ablation.Scientific reports · 2022Article
- Review
- Caveolin-1 is Involved in Regulating the Biological Response of Cells to Nanosecond Pulsed Electric Fields.The Journal of membrane biology · 2021Article
- Article
- Nanosecond pulsed electric fields induce extracellular release of chromosomal DNA and histone citrullination in neutrophil-differentiated HL-60 cells.Scientific reports · 2019Article
- Mechanisms and immunogenicity of nsPEF-induced cell death in B16F10 melanoma tumors.Scientific reports · 2019Article
- Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks.Scientific reports · 2018Article
- Asymmetrical bipolar nanosecond electric pulse widths modify bipolar cancellation.Scientific reports · 2017Article
- Selective susceptibility to nanosecond pulsed electric field (nsPEF) across different human cell types.Cellular and molecular life sciences : CMLS · 2017Article
- Effect of Cooling On Cell Volume and Viability After Nanoelectroporation.The Journal of membrane biology · 2017Article
- Adult human dermal fibroblasts exposed to nanosecond electrical pulses exhibit genetic biomarkers of mechanical stress.Biochemistry and biophysics reports · 2017Article
- Exploring the Applicability of Nano-Poration for Remote Control in Smart Drug Delivery Systems.The Journal of membrane biology · 2017Article
- Technological and Theoretical Aspects for Testing Electroporation on Liposomes.BioMed research international · 2017Article
- The cytotoxic synergy of nanosecond electric pulses and low temperature leads to apoptosis.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
In this study, we determined the LD(50) (50% lethal dose) for cell death, and the ED(50) (50% of cell population staining positive) for propidium (Pr) iodide uptake, and phosphatidylserine (PS) externalization for several commonly studied cell lines (HeLa, Jurkat, U937, CHO-K1, and GH3) exposed to 10-ns electric pulses (EP). We found that the LD(50) varied substantially across the cell lines studied, increasing from 51 J/g for Jurkat to 1861 J/g for HeLa. PS externalized at doses equal or lower than that required for death in all cell lines ranging from 51 J/g in Jurkat, to 199 J/g in CHO-K1. Pr uptake occurred at doses lower than required for death in three of the cell lines: 656 J/g for CHO-K1, 634 J/g for HeLa, and 142 J/g for GH3. Both Jurkat and U937 had a LD(50) lower than the ED(50) for Pr uptake at 780 J/g and 1274 J/g, respectively. The mechanism responsible for these differences was explored by evaluating cell size, calcium concentration in the exposure medium, and effect of trypsin treatment prior to exposure. None of the studied parameters correlated with the observed results suggesting that cellular susceptibility to injury and death by 10-ns EP was largely determined by cell physiology. In contrast to previous studies, our findings suggest that permeabilization of internal membranes may not necessarily be responsible for cell death by 10-ns EP. Additionally, a mixture of Jurkat and HeLa cells was exposed to 10-ns EP at a dose of 280 J/g. Death was observed only in Jurkat cells suggesting that 10-ns EP may selectively kill cells within a heterogeneous tissue.
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Registered trials
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