ArticleCell death & disease2011
NPR-A regulates self-renewal and pluripotency of embryonic stem cells.
Article in Cell death & disease, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.
- Molecular Mechanisms Underlying Pluripotency and Self-Renewal of Embryonic Stem Cells.International journal of molecular sciences · 2023Pooled it
- ANP Increases ZnCurrent medical science · 2025Article
- Regulation and Pharmacology of the Cyclic GMP and Nitric Oxide Pathway in Embryonic and Adult Stem Cells.Cells · 2024Review
- Developmental roles of natriuretic peptides and their receptors.Cells & development · 2023Review
- Article
- Is Atrial Natriuretic Peptide (ANP) and Natriuretic Peptide Receptor-A (NPR-A) Expression in Human Placenta and Decidua Normal?Medical science monitor : international medical journal of experimental and clinical research · 2019Article
- Article
- Localization of Brain Natriuretic Peptide Immunoreactivity in Rat Spinal Cord.Frontiers in neuroanatomy · 2016Article
- Pharmacological Therapy in the Heart as an Alternative to Cellular Therapy: A Place for the Brain Natriuretic Peptide?Stem cells international · 2016Review
- Gigantol Suppresses Cancer Stem Cell-Like Phenotypes in Lung Cancer Cells.Evidence-based complementary and alternative medicine : eCAM · 2015Article
- Sox2 gene amplification significantly impacts overall survival in serous epithelial ovarian cancer.Reproductive sciences (Thousand Oaks, Calif.) · 2015Article
- New proteomic insights on the role of NPR-A in regulating self-renewal of embryonic stem cells.Stem cell reviews and reports · 2014Article
- Natriuretic peptide receptor A as a novel target for cancer.World journal of surgical oncology · 2014Review
- Impact of NPR-A expression in gastric cancer cells.International journal of clinical and experimental medicine · 2014Article
- Molecular mechanisms controlling the cell cycle in embryonic stem cells.Stem cell reviews and reports · 2013Review
- Atrial natriuretic peptide modulates the proliferation of human gastric cancer cells via KCNQ1 expression.Oncology letters · 2013Article
- Suppression of atrial natriuretic peptide/natriuretic peptide receptor-A-mediated signaling upregulates angiotensin-II-induced collagen synthesis in adult cardiac fibroblasts.Molecular and cellular biochemistry · 2013Article
- Oct4 promoter activity in stem cells obtained through somatic reprogramming.Cellular reprogramming · 2013Article
- B-type natriuretic peptide-induced delayed modulation of TRPV1 and P2X3 receptors of mouse trigeminal sensory neurons.PloS one · 2013Article
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Self-renewal and pluripotency of embryonic stem (ES) cells are maintained by several signaling cascades and by expression of intrinsic factors, such as Oct4, Nanog and Sox2. The mechanism regulating these signaling cascades in ES cells is of great interest. Recently, we have demonstrated that natriuretic peptide receptor A (NPR-A), a specific receptor for atrial and brain natriuretic peptides (ANP and BNP, respectively), is expressed in pre-implantation embryos and in ES cells. Here, we examined whether NPR-A is involved in the maintenance of ES cell pluripotency. RNA interference-mediated knockdown of NPR-A resulted in phenotypic changes, indicative of differentiation, downregulation of pluripotency factors (such as Oct4, Nanog and Sox2) and upregulation of differentiation genes. NPR-A knockdown also resulted in a marked downregulation of phosphorylated Akt. Furthermore, NPR-A knockdown induced accumulation of ES cells in the G1 phase of the cell cycle. Interestingly, we found that ANP was expressed in self-renewing ES cells, whereas its level was reduced after ES cell differentiation. Treatment of ES cells with ANP upregulated the expression of Oct4, Nanog and phosphorylated Akt, and this upregulation depended on NPR-A signaling, because it was completely reversed by pretreatment with either an NPR-A antagonist or a cGMP-dependent protein kinase inhibitor. These findings provide a novel role for NPR-A in the maintenance of self-renewal and pluripotency of ES cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.