ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2011
Experimental protocols alter phototransduction: the implications for retinal processing at visual threshold.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- The effects of bicarbonate on the aberrant photon response in murine rod photoreceptors.Biophysical journal · 2026Article
- S-cone-specific circuitry in the outer plexiform layer of a cone-dominant mammal.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- An Ex Vivo Electroretinographic Apparatus for the mL-Scale Testing of Drugs to One Day and Beyond.International journal of molecular sciences · 2023Article
- Light responses of mammalian cones.Pflugers Archiv : European journal of physiology · 2021Review
- A kinetic analysis of mouse rod and cone photoreceptor responses.The Journal of physiology · 2020Article
- Submicromolar copper (II) ions stimulate transretinal signaling in the isolated retina from wild type but not from CaBMC ophthalmology · 2020Article
- Sensitivity of Mammalian Cone Photoreceptors to Infrared Light.Neuroscience · 2019Article
- Rod Photoresponse Kinetics Limit Temporal Contrast Sensitivity in Mesopic Vision.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Rods progressively escape saturation to drive visual responses in daylight conditions.Nature communications · 2017Article
- Biophysical Variation within the M1 Type of Ganglion Cell Photoreceptor.Cell reports · 2017Article
- Bright flash response recovery of mammalian rods in vivo is rate limited by RGS9.The Journal of general physiology · 2017Article
- A novel Ca2+-feedback mechanism extends the operating range of mammalian rods to brighter light.The Journal of general physiology · 2015Article
- A Cambrian origin for vertebrate rods.eLife · 2015Article
- Simultaneous ex vivo functional testing of two retinas by in vivo electroretinogram system.Journal of visualized experiments : JoVE · 2015Article
- Speeding rod recovery improves temporal resolution in the retina.Vision research · 2015Article
- Article
- Ophthalmic Manifestations of Amyotrophic Lateral Sclerosis (An American Ophthalmological Society Thesis).Transactions of the American Ophthalmological Society · 2015Observational
- Adaptive potentiation in rod photoreceptors after light exposure.The Journal of general physiology · 2014Article
- Light responses of primate and other mammalian cones.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Vision in dim light, when photons are scarce, requires reliable signaling of the arrival of single photons. Rod photoreceptors accomplish this task through the use of a G-protein-coupled transduction cascade that amplifies the activity of single active rhodopsin molecules. This process is one of the best understood signaling cascades in biology, yet quantitative measurements of the amplitude and kinetics of the rod's response in mice vary by a factor of ∼ 2 across studies. What accounts for these discrepancies? We used several experimental approaches to reconcile differences in published properties of rod responses. First, we used suction electrode recordings from single rods to compare measurements across a range of recording conditions. Second, we compared measurements of single-cell photocurrents to estimates of rod function from in vitro electroretinograms. Third, we assayed the health of the post-receptor retinal tissue in these different conditions. Several salient points emerge from these experiments: (1) recorded responses can be altered dramatically by how the retina is stored; (2) the kinetics of the recovery of responses to bright but not dim flashes are strongly sensitive to the extracellular concentration of magnesium; (3) experimental conditions that produce very different single-photon responses measured in single rods produce near identical derived rod responses from the electroretinogram. The dependence of rod responses on experimental conditions will be a key consideration in efforts to extract general principles of G-protein signaling from studies of phototransduction and to relate these signals to downstream mechanisms that facilitate visual sensitivity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.