ArticleJournal of clinical microbiology2011
Clinical relevance of multiple single-nucleotide polymorphisms in Pneumocystis jirovecii Pneumonia: development of a multiplex PCR-single-base-extension methodology.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Multi-site screening forFrontiers in cellular and infection microbiology · 2026Article
- Axenic Long-Term Cultivation ofJournal of fungi (Basel, Switzerland) · 2023Article
- A Real-Time PCR Assay for Detection of LowFrontiers in microbiology · 2021Article
- Diagnosing Pneumocystis jirovecii pneumonia: A review of current methods and novel approaches.Medical mycology · 2020Review
- Outbreak-Causing Fungi: Pneumocystis jirovecii.Mycopathologia · 2020Review
- Article
- Comparison of MultiLocus Sequence Typing (MLST) and Microsatellite Length Polymorphism (MLP) forComputational and structural biotechnology journal · 2020Article
- Genetic Polymorphisms of Superoxide Dismutase Locus ofFrontiers in public health · 2019Article
- A Molecular Window into the Biology and Epidemiology of Pneumocystis spp.Clinical microbiology reviews · 2018Review
- Pneumocystis jirovecii and Cystic Fibrosis in Brittany, France.Mycopathologia · 2018Article
- Diversity of Pneumocystis jirovecii Across Europe: A Multicentre Observational Study.EBioMedicine · 2017Observational
- Prevalence of colonization and mitochondrial large subunit rRNA mutation ofIranian journal of microbiology · 2016Article
- Diversity of Pneumocystis jirovecii during Infection Revealed by Ultra-Deep Pyrosequencing.Frontiers in microbiology · 2016Article
- Pneumocystis jirovecii--from a commensal to pathogen: clinical and diagnostic review.Parasitology research · 2015Review
- Article
- (1-3)-beta-D-glucan in association with lactate dehydrogenase as biomarkers of Pneumocystis pneumonia (PcP) in HIV-infected patients.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2014Article
- Multilocus microsatellite genotyping array for investigation of genetic epidemiology of Pneumocystis jirovecii.Journal of clinical microbiology · 2014Article
- Prevalence and genotype distribution of Pneumocystis jirovecii in Cuban infants and toddlers with whooping cough.Journal of clinical microbiology · 2014Article
- Trimethoprim resistance of dihydrofolate reductase variants from clinical isolates of Pneumocystis jirovecii.Antimicrobial agents and chemotherapy · 2013Article
- Pneumocystis jirovecii genotype associated with increased death rate of HIV-infected patients with pneumonia.Emerging infectious diseases · 2013Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pneumocystis jirovecii pneumonia (PcP) is a major cause of respiratory illness in patients with AIDS. The identification of multiple single-nucleotide polymorphisms (SNPs) at three distinct P. jirovecii loci encoding dihydrofolate reductase (DHFR), mitochondrial large-subunit rRNA (mtLSU rRNA), and superoxide dismutase (SOD) was achieved using multiplex-PCR (MPCR) followed by direct sequencing and two single-base extension (SBE) techniques. Four SNPs (DHFR312, mt85, SOD215, and SOD110), correlated previously with parameters of disease, were amplified and genotyped simultaneously. The concordance of results between the standard sequencing technique (direct sequencing) and SBE analysis was 96.9% for the acrylamide gel electrophoresis and 98.4% for the capillary electrophoresis. The cross-genetic analysis established several statistical associations among the SNPs studied: mt85C-SOD110T, SOD110T-SOD215C, and SOD110C-SOD215T. These results were confirmed by cluster analysis. Data showed that among the isolates with low to moderate parasite burden, the highest percentages of DHFR312C, mt85C, SOD110T, and SOD215C were detected, whereas for high parasite burden cases the highest frequencies were observed among isolates with DHFR312T, mt85T, SOD110C, and SOD215T. The polymorphisms studied were shown to be suitable genetic targets potentially correlated with PcP clinical data that can be used as predictors of outcome in further studies to help clinical decision-making in the management of PcP. The MPCR/SBE protocol described for the first time in the present study was shown to be a rapid, highly accurate method for genotyping P. jirovecii SNPs encoded by different loci that could be used for epidemiological studies and as an additional procedure for the prognostic classification and diagnosis of PcP.
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