ArticleJournal of virology2011
Ribosome binding to a 5' translational enhancer is altered in the presence of the 3' untranslated region in cap-independent translation of turnip crinkle virus.
Article in Journal of virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 59 citations in OpenAlex.
- Identification and Genome Characterization of a Novel Nege-like Virus Isolated from Aphids (International journal of molecular sciences · 2024Article
- Modeling alternative translation initiation sites in plants reveals evolutionarily conservedGenome research · 2024Article
- Advances in Understanding the Mechanism of Cap-Independent Cucurbit Aphid-Borne Yellows Virus Protein Synthesis.International journal of molecular sciences · 2023Article
- Crystal structure of a cap-independent translation enhancer RNA.Nucleic acids research · 2023Article
- Conserved Structure Associated with Different 3'CITEs Is Important for Translation of Umbraviruses.Viruses · 2023Article
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- Conserved RNA secondary structure in Cherry virus A 5'-UTR associated with translation regulation.Virology journal · 2022Article
- Identification of Novel 5' and 3' Translation Enhancers in Umbravirus-Like Coat Protein-Deficient RNA Replicons.Journal of virology · 2022Article
- Structural characterization of a new subclass of panicum mosaic virus-like 3' cap-independent translation enhancer.Nucleic acids research · 2022Article
- MultipleFrontiers in plant science · 2022Article
- Non-Canonical Translation Initiation Mechanisms Employed by Eukaryotic Viral mRNAs.Biochemistry. Biokhimiia · 2021Review
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- The 3' Untranslated Region of a Plant Viral RNA Directs Efficient Cap-Independent Translation in Plant and Mammalian Systems.Pathogens (Basel, Switzerland) · 2019Article
- RNA virus evasion of nonsense-mediated decay.PLoS pathogens · 2018Article
- Article
- A Dual Interaction Between the 5'- and 3'-Ends of the Melon Necrotic Spot Virus (MNSV) RNA Genome Is Required for Efficient Cap-Independent Translation.Frontiers in plant science · 2018Article
- Article
- Concerted action of two 3' cap-independent translation enhancers increases the competitive strength of translated viral genomes.Nucleic acids research · 2017Article
- A Sequence-Independent, Unstructured Internal Ribosome Entry Site Is Responsible for Internal Expression of the Coat Protein of Turnip Crinkle Virus.Journal of virology · 2017Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Plus-strand RNA viruses without 5' caps require noncanonical mechanisms for ribosome recruitment. A translational enhancer in the 3' untranslated region (UTR) of Turnip crinkle virus (TCV) contains an internal T-shaped structure (TSS) that binds to 60S ribosomal subunits. We now report that the 63-nucleotide (nt) 5' UTR of TCV contains a 19-nt pyrimidine-rich element near the initiation codon that supports translation of an internal open reading frame (ORF) independent of upstream 5' UTR sequences. Addition of 80S ribosomes to the 5' UTR reduced the flexibility of the polypyrimidine residues and generated a toeprint consistent with binding to this region. Binding of salt-washed 40S ribosomal subunits was reduced 6-fold when the pyrimidine-rich sequence was mutated. 40S subunit binding generated the same toeprint as 80S ribosomes but also additional ones near the 5' end. Generation of out-of-frame AUGs upstream of the polypyrimidine region reduced translation, which suggests that 5'-terminal entry of 40S subunits is followed by scanning and that the polypyrimidine region is needed for an alternative function that requires ribosome binding. No evidence for RNA-RNA interactions between 5' and 3' sequences was found, suggesting that TCV utilizes an alternative means for circularizing its genome. Combining 5' and 3' UTR fragments in vitro had no discernible effect on the structures of the RNAs. In contrast, when 80S ribosomes were added to both fragments, structural changes were found in the 5' UTR polypyrimidine tract that were not evident when ribosomes interacted with the individual fragments. This suggests that ribosomes can promote an interaction between the 5' and 3' UTRs of TCV.
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