ArticleJournal of magnetic resonance (San Diego, Calif. : 1997)2011
Pulsed dipolar spectroscopy distance measurements in biomacromolecules labeled with Gd(III) markers.
Article in Journal of magnetic resonance (San Diego, Calif. : 1997), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Caught in the Middle: A Rigid DNA Label That Provides an Incisive Picture of DNA Conformational Flexibility in Protein-DNA Complexes.Journal of the American Chemical Society · 2025Article
- Robustness and Sensitivity of Gd(III)-Gd(III) Double Electron-Electron Resonance (DEER) Measurements: Comparative Study of High-Frequency EPR Spectrometer Designs and Spin Label Variants.Applied magnetic resonance · 2025Article
- DEER and RIDME Measurements of the Nitroxide-Spin Labelled Copper-Bound Amine Oxidase Homodimer from Arthrobacter Globiformis.Applied magnetic resonance · 2021Article
- Orientation and dynamics of CuPhysical chemistry chemical physics : PCCP · 2020Article
- Cu2+-based distance measurements by pulsed EPR provide distance constraints for DNA backbone conformations in solution.Nucleic acids research · 2020Article
- Probing the solution structure of the E. coli multidrug transporter MdfA using DEER distance measurements with nitroxide and Gd(III) spin labels.Scientific reports · 2019Article
- GdPhysical chemistry chemical physics : PCCP · 2017Article
- Pulse EPR-enabled interpretation of scarce pseudocontact shifts induced by lanthanide binding tags.Journal of biomolecular NMR · 2016Article
- Encoded loop-lanthanide-binding tags for long-range distance measurements in proteins by NMR and EPR spectroscopy.Journal of biomolecular NMR · 2015Article
- Zero field splitting fluctuations induced phase relaxation of Gd3+ in frozen solutions at cryogenic temperatures.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2014Article
- New developments in spin labels for pulsed dipolar EPR.Molecules (Basel, Switzerland) · 2014Review
- Copper-based pulsed dipolar ESR spectroscopy as a probe of protein conformation linked to disease states.Biophysical journal · 2014Article
- Pulsed EPR distance measurements in soluble proteins by site-directed spin labeling (SDSL).Current protocols in protein science · 2013Article
- mtsslSuite:Molecular physics · 2013Article
- Optimization of pulsed DEER measurements for Gd-based labels: choice of operational frequencies, pulse durations and positions, and temperature.Applied magnetic resonance · 2013Article
- Pulsed electron double resonance in structural studies of spin-labeled nucleic acids.Acta naturae · 2013Article
- Lanthanide binding and IgG affinity construct: potential applications in solution NMR, MRI, and luminescence microscopy.Protein science : a publication of the Protein Society · 2012Article
- Magnetic resonance tumor targeting imaging using gadolinium labeled human telomerase reverse transcriptase antisense probes.Cancer science · 2012Article
- Dielectric Resonator forApplied magnetic resonance · 2012Article
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6 authors.
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Abstract
This work demonstrates the feasibility of using Gd(III) tags for long-range Double Electron Electron Resonance (DEER) distance measurements in biomacromolecules. Double-stranded 14- base pair Gd(III)-DNA conjugates were synthesized and investigated at K(a) band. For the longest Gd(III) tag the average distance and average deviation between Gd(III) ions determined from the DEER time domains was about 59±12Å. This result demonstrates that DEER measurements with Gd(III) tags can be routinely carried out for distances of at least 60Å, and analysis indicates that distance measurements up to 100Å are possible. Compared with commonly used nitroxide labels, Gd(III)-based labels will be most beneficial for the detection of distance variations in large biomacromolecules, with an emphasis on large scale changes in shape or distance. Tracking the folding/unfolding and domain interactions of proteins and the conformational changes in DNA are examples of such applications.
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