ArticleBiomaterials2011
Dynamic quantitative visualization of single cell alignment and migration and matrix remodeling in 3-D collagen hydrogels under mechanical force.
Article in Biomaterials, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
26 citing papers in PubMed, 50 citations in OpenAlex.
- Cyclic stretch inhibits cell invasion in 3D scaffolds.bioRxiv : the preprint server for biology · 2026Article
- Towards a more objective and high-throughput spheroid invasion assay quantification method.Scientific reports · 2024Article
- Towards a More Objective and High-throughput Spheroid Invasion Assay Quantification Method.bioRxiv : the preprint server for biology · 2024Article
- A Review on the Applications of Natural Biodegradable Nano Polymers in Cardiac Tissue Engineering.Nanomaterials (Basel, Switzerland) · 2023Review
- Design and aligner-assisted fast fabrication of a microfluidic platform for quasi-3D cell studies on an elastic polymer.Bioactive materials · 2022Article
- Extracellular Matrix Alignment Directs Provisional Matrix Assembly and Three Dimensional Fibrous Tissue Closure.Tissue engineering. Part A · 2021Article
- Mechano-chemo signaling interactions modulate matrix production by cardiac fibroblasts.Matrix biology plus · 2021Article
- Processing advances in liquid crystal elastomers provide a path to biomedical applications.Journal of applied physics · 2020Article
- Microfluidic fabrication of stable collagen microgels with aligned microstructure using flow-driven co-deposition and ionic gelation.Journal of micromechanics and microengineering : structures, devices, and systems · 2020Article
- Optimizing Blended Collagen-Fibrin Hydrogels for Cardiac Tissue Engineering with Human iPSC-derived Cardiomyocytes.ACS biomaterials science & engineering · 2019Article
- Role of boundary conditions in determining cell alignment in response to stretch.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Mechanoregulation of Myofibroblast Fate and Cardiac Fibrosis.Advanced biosystems · 2018Article
- Cell Contractility Facilitates Alignment of Cells and Tissues to Static Uniaxial Stretch.Biophysical journal · 2017Article
- Local alignment vectors reveal cancer cell-induced ECM fiber remodeling dynamics.Scientific reports · 2017Article
- Magnitude-dependent proliferation and contractility modulation of human bladder smooth muscle cells under physiological stretch.World journal of urology · 2015Article
- Dynamic tensile forces drive collective cell migration through three-dimensional extracellular matrices.Scientific reports · 2015Article
- Metastatic bladder cancer cells distinctively sense and respond to physical cues of collagen fibril-mimetic nanotopography.Experimental biology and medicine (Maywood, N.J.) · 2015Article
- Effects of substrate conductivity on cell morphogenesis and proliferation using tailored, atomic layer deposition-grown ZnO thin films.Scientific reports · 2015Article
- Rapid Quantification of 3D Collagen Fiber Alignment and Fiber Intersection Correlations with High Sensitivity.PloS one · 2015Article
- Modeling persistence in mesenchymal cell motility using explicit fibers.Langmuir : the ACS journal of surfaces and colloids · 2014Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
We developed a live imaging system enabling dynamic visualization of single cell alignment induced by external mechanical force in a 3-D collagen matrix. The alignment dynamics and migration of smooth muscle cells (SMCs) were studied by time lapse differential interference contrast and/or phase contrast microscopy. Fluorescent and reflection confocal microcopy were used to study the SMC morphology and the microscale collagen matrix remodeling induced by SMCs. A custom developed program was used to quantify the cell migration and matrix remodeling. Our system enables cell concentration-independent alignment eliminating cell-to-cell interference and enables dynamic cell tracking, high magnification observation and rapid cell alignment accomplished in a few hours compared to days in traditional models. We observed that cells sense and response to the mechanical signal before cell spreading. Under mechanical stretch the migration directionality index of SMCs is 46.3% more than those cells without external stretch; the dynamic direction of cell protrusion is aligned to that of the mechanical force; SMCs showed directional matrix remodeling and the alignment index calculated from the matrix in front of cell protrusions is about 3 fold of that adjacent to cell bodies. Our results indicate that the mechanism of cell alignment is directional cell protrusion. Mechano-sensing, directionality in cell protrusion dynamics, cell migration and matrix remodeling are highly integrated. Our system provides a platform for studying the role of mechanical force on the cell matrix interactions and thus finds strategies to optimize selected properties of engineered tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.