Evidence map›Paper›PMID 21387148›Full record

ArticlePharmaceutical research2011

Enhanced stability and knockdown efficiency of poly(ethylene glycol)-b-polyphosphoramidate/siRNA micellar nanoparticles by co-condensation with sodium triphosphate.

Masataka Nakanishi, Rajesh Patil, Yong Ren, Rishab Shyam, Philip Wong, Hai Quan Mao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pharmaceutical research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Evaluation of Poly(Pharmaceutics · 2023
    Article
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Masataka Nakanishi *Department of Materials Science and Engineering Johns Hopkins University Baltimore, Maryland 21218, USA Translational Tissue Engineering Center, Johns Hopkins University Baltimore, Maryland 21218, USA Department of Biomedical Engineering, Johns Hopkins University Baltimore, Maryland 21218, USA Department of Neuroscience, Johns Hopkins University Baltimore, Maryland 21218, USA Whitaker Biomedical Engineering Institute, Johns Hopkins University Baltimore, Maryland 21218, USA 101E Maryland Hall, 3400 North Charles Street Baltimore, Maryland 21218, USA.
Rajesh Patil *Department of Materials Science and Engineering Johns Hopkins University Baltimore, Maryland 21218, USA Translational Tissue Engineering Center, Johns Hopkins University Baltimore, Maryland 21218, USA Department of Biomedical Engineering, Johns Hopkins University Baltimore, Maryland 21218, USA Department of Neuroscience, Johns Hopkins University Baltimore, Maryland 21218, USA Whitaker Biomedical Engineering Institute, Johns Hopkins University Baltimore, Maryland 21218, USA 101E Maryland Hall, 3400 North Charles Street Baltimore, Maryland 21218, USA.
Yong RenDepartment of Materials Science and Engineering Johns Hopkins University Baltimore, Maryland 21218, USA Translational Tissue Engineering Center, Johns Hopkins University Baltimore, Maryland 21218, USA Department of Biomedical Engineering, Johns Hopkins University Baltimore, Maryland 21218, USA Department of Neuroscience, Johns Hopkins University Baltimore, Maryland 21218, USA Whitaker Biomedical Engineering Institute, Johns Hopkins University Baltimore, Maryland 21218, USA 101E Maryland Hall, 3400 North Charles Street Baltimore, Maryland 21218, USA.
Rishab ShyamDepartment of Materials Science and Engineering Johns Hopkins University Baltimore, Maryland 21218, USA Translational Tissue Engineering Center, Johns Hopkins University Baltimore, Maryland 21218, USA Department of Biomedical Engineering, Johns Hopkins University Baltimore, Maryland 21218, USA Department of Neuroscience, Johns Hopkins University Baltimore, Maryland 21218, USA Whitaker Biomedical Engineering Institute, Johns Hopkins University Baltimore, Maryland 21218, USA 101E Maryland Hall, 3400 North Charles Street Baltimore, Maryland 21218, USA.
Philip WongDepartment of Materials Science and Engineering Johns Hopkins University Baltimore, Maryland 21218, USA Translational Tissue Engineering Center, Johns Hopkins University Baltimore, Maryland 21218, USA Department of Biomedical Engineering, Johns Hopkins University Baltimore, Maryland 21218, USA Department of Neuroscience, Johns Hopkins University Baltimore, Maryland 21218, USA Whitaker Biomedical Engineering Institute, Johns Hopkins University Baltimore, Maryland 21218, USA 101E Maryland Hall, 3400 North Charles Street Baltimore, Maryland 21218, USA.
Hai Quan MaoDepartment of Materials Science and Engineering Johns Hopkins University Baltimore, Maryland 21218, USA Translational Tissue Engineering Center, Johns Hopkins University Baltimore, Maryland 21218, USA Department of Biomedical Engineering, Johns Hopkins University Baltimore, Maryland 21218, USA Department of Neuroscience, Johns Hopkins University Baltimore, Maryland 21218, USA Whitaker Biomedical Engineering Institute, Johns Hopkins University Baltimore, Maryland 21218, USA 101E Maryland Hall, 3400 North Charles Street Baltimore, Maryland 21218, USA.

Funding

'T V ASSESSMENT - MEMORY DISORDERED PATIENTS'P50AG005146 · NIA · JOHNS HOPKINS UNIVERSITY · PI HUGANIR, RICHARD L · 1985 to 2019
$37.6M
NIA NIH HHS P50 AG005146
6 · The paper itself

Abstract

purposePolyelectrolyte complex nanoparticles are a promising vehicle for siRNA delivery but suffer from low stability under physiological conditions. An effective stabilization method is essential for the success of polycationic nanoparticle-mediated siRNA delivery. In this study, sodium triphosphate (TPP), an ionic crosslinking agent, is used to stabilize siRNA-containing nanoparticles by co-condensation.

methodssiRNA and TPP were co-encapsulated into a block copolymer, poly(ethylene glycol)-b-polyphosphoramidate (PEG-b-PPA), to form ternary nanoparticles. Physicochemical characterization was performed by dynamic light scattering and gel electrophoresis. Gene silencing efficiency in cell lines was assessed by dual luciferase assay system.

resultsThe PEG-b-PPA/siRNA/TPP ternary nanoparticles exhibited high uniformity with smaller size (80-100 nm) compared with PEG-b-PPA/siRNA nanoparticles and showed increased stability in physiological ionic strength and serum-containing medium, due to the stabilization effect from ionic crosslinks between negatively charged TPP and cationic PPA segment. Transfection and gene silencing efficiency of the TPP-crosslinked nanoparticles were markedly improved over PEG-b-PPA/siRNA complexes in serum-containing medium. No significant difference in cell viability was observed between nanoparticles prepared with and without TPP co-condensation.

conclusionsThese results demonstrated the effectiveness of TPP co-condensation in compacting polycation/siRNA nanoparticles, improving nanoparticle stability and enhancing the transfection and knockdown efficiency in serum-containing medium.

Indexed as

MicellesAmidesAnionsCell SurvivalDrug StabilityGene SilencingHeLa CellsHumansMicroscopy, Electron, TransmissionModels, BiologicalNanoparticlesParticle SizePhosphoric AcidsPolyethylene GlycolsPolyphosphatesRNA, Small InterferingAmidesAnionsMicellesphosphoramidic acidPhosphoric AcidsPolyethylene GlycolsPolyphosphatesRNA, Small Interferingtriphosphoric acid

Identifiers

PMID21387148
PMCPMC4030395

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.