ArticlePloS one2011
Identification of the transcriptional regulator NcrB in the nickel resistance determinant of Leptospirillum ferriphilum UBK03.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Bacterial Metallostasis: Metal Sensing, Metalloproteome Remodeling, and Metal Trafficking.Chemical reviews · 2024Review
- Functional asymmetry and chemical reactivity of CsoR family persulfide sensors.Nucleic acids research · 2021Article
- Nickel Metalloregulators and Chaperones.Inorganics · 2019Article
- Pb2+ tolerance by Frankia sp. strain EAN1pec involves surface-binding.Microbiology (Reading, England) · 2017Article
- Two draft genome sequences ofStandards in genomic sciences · 2016Article
- Generating a Metal-responsive Transcriptional Regulator to Test What Confers Metal Sensing in Cells.The Journal of biological chemistry · 2015Article
- Cu(I)-mediated allosteric switching in a copper-sensing operon repressor (CsoR).The Journal of biological chemistry · 2014Article
- Metal specificity of cyanobacterial nickel-responsive repressor InrS: cells maintain zinc and copper below the detection threshold for InrS.Molecular microbiology · 2014Article
- Nickel-resistance determinants in Acidiphilium sp. PM identified by genome-wide functional screening.PloS one · 2014Article
- Specific metal recognition in nickel trafficking.Biochemistry · 2012Review
- Cytosolic Ni(II) sensor in cyanobacterium: nickel detection follows nickel affinity across four families of metal sensors.The Journal of biological chemistry · 2012Article
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Authors and funding
5 authors.
Funding
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Abstract
The nickel resistance determinant ncrABCY was identified in Leptospirillum ferriphilum UBK03. Within this operon, ncrA and ncrC encode two membrane proteins that form an efflux system, and ncrB encodes NcrB, which belongs to an uncharacterized family (DUF156) of proteins. How this determinant is regulated remains unknown. Our data indicate that expression of the nickel resistance determinant is induced by nickel. The promoter of ncrA, designated pncrA, was cloned into the promoter probe vector pPR9TT, and co-transformed with either a wild-type or mutant nickel resistance determinant. The results revealed that ncrB encoded a transcriptional regulator that could regulate the expression of ncrA, ncrB, and ncrC. A GC-rich inverted repeat sequence was identified in the promoter pncrA. Electrophoretic mobility shift assays (EMSAs) and footprinting assays showed that purified NcrB could specifically bind to the inverted repeat sequence of pncrA in vitro; this was confirmed by bacterial one-hybrid analysis. Moreover, this binding was inhibited in the presence of nickel ions. Thus, we classified NcrB as a transcriptional regulator that recognizes the inverted repeat sequence binding motif to regulate the expression of the key nickel resistance gene, ncrA.
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