ArticlePloS one2011
Cell-cycle analysis of fission yeast cells by flow cytometry.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 55 citations in OpenAlex.
- Article
- Investigating Fission Yeast Mutagenesis Using Canavanine Sensitivity Assays.Methods in molecular biology (Clifton, N.J.) · 2025Article
- A Rapidly Inducible DNA Double-Strand Break to Monitor Telomere Formation, DNA Repair, and Checkpoint Activation.Methods in molecular biology (Clifton, N.J.) · 2025Article
- The Rtf1/Prf1-dependent histone modification axis counteracts multi-drug resistance in fission yeast.Life science alliance · 2024Article
- Article
- Detecting Cell Cycle Stage and Progression in Fission Yeast, Schizosaccharomyces pombe.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The histone chaperone FACT facilitates heterochromatin spreading by regulating histone turnover and H3K9 methylation states.Cell reports · 2021Article
- High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.International journal of molecular sciences · 2020Article
- Article
- The TOR pathway modulates cytoophidium formation inThe Journal of biological chemistry · 2019Article
- Histone H2B Ubiquitylation Regulates Histone Gene Expression by Suppressing Antisense Transcription in Fission Yeast.Genetics · 2019Article
- Article
- A novel role for ATR/Rad3 in G1 phase.Scientific reports · 2018Article
- Phosphorylation of the amino-terminus of the AGC kinase Gad8 prevents its interaction with TORC2.Open biology · 2016Article
- Stress-induced inhibition of translation independently of eIF2α phosphorylation.Journal of cell science · 2015Article
- Genome-Wide Estimates of Mutation Rates and Spectrum in Schizosaccharomyces pombe Indicate CpG Sites are Highly Mutagenic Despite the Absence of DNA Methylation.G3 (Bethesda, Md.) · 2015Article
- Evaluating the Toxicity of the Analgesic Glutaminase Inhibitor 6-Diazo-5-Oxo-L-NorleucineMOJ toxicology · 2015Article
- Fission Yeast CSL Transcription Factors: Mapping Their Target Genes and Biological Roles.PloS one · 2015Article
- Article
- DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast.Molecular biology of the cell · 2012Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cell cycle of the fission yeast, Schizosaccharomyces pombe, does not easily lend itself to analysis by flow cytometry, mainly because cells in G(1) and G(2) phase contain the same amount of DNA. This occurs because fission yeast cells under standard growth conditions do not complete cytokinesis until after G(1) phase. We have devised a flow cytometric method exploiting the fact that cells in G(1) phase contain two nuclei, whereas cells in G(2) are mononuclear. Measurements of the width as well as the total area of the DNA-associated fluorescence signal allows the discrimination between cells in G(1) and in G(2) phase and the cell-cycle progression of fission yeast can be followed in detail by flow cytometry. Furthermore, we show how this method can be used to monitor the timing of cell entry into anaphase. Fission yeast cells tend to form multimers, which represents another problem of flow cytometry-based cell-cycle analysis. Here we present a method employing light-scatter measurements to enable the exclusion of cell doublets, thereby further improving the analysis of fission yeast cells by flow cytometry.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.