ArticleThe Plant cell2011
Phosphorylation of the Nicotiana benthamiana WRKY8 transcription factor by MAPK functions in the defense response.
Article in The Plant cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 119 papers.
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Who cites it
119 citing papers in PubMed, 267 citations in OpenAlex.
- How WRKY transcription factors fine-tune specificity in plant stress responses: from W-box to regulatory code.Molecular biology reports · 2026Review
- The MPK6-WRKY49 module links MAPK signaling to ABA biosynthesis and drought tolerance in Arabidopsis.The Plant journal : for cell and molecular biology · 2026Article
- Jasmonate Modulates Strawberry Susceptibility to Anthracnose by Activating SnRK2.1 to Regulate the WRKY50-JAZ5 Module.Plant biotechnology journal · 2026Article
- The Jasmonate-Responsive SmMPK3-SmWRKY33 Module Positively Regulates Tanshinone Biosynthesis in Salvia miltiorrhiza.Plant biotechnology journal · 2026Article
- Review
- Molecular changes in agroinfiltrated leaves of Nicotiana benthamiana expressing suppressor of silencing P19 and coronavirus-like particles.Plant biotechnology journal · 2026Article
- Putative Transcriptional Regulation of HaWRKY33-AOA251SVV7 Complex-Mediated Sunflower Head Rot by Transcriptomics and Proteomics.Plants (Basel, Switzerland) · 2025Article
- Interaction Between Glycoside Hydrolase FsGH28c fromInternational journal of molecular sciences · 2025Article
- Transient Overexpression of the PepperLife (Basel, Switzerland) · 2025Article
- From Recognition to Response: Resistance-Effector Gene Interactions in thePlants (Basel, Switzerland) · 2025Review
- Two leucine-rich repeat receptor-like kinases initiate herbivory defense responses in tea plants.Horticulture research · 2025Article
- Towards sustainable agarwood production: integrating microbial interactions, anatomical changes, and metabolite biosynthesis.Journal of industrial microbiology & biotechnology · 2024Review
- CDPK5 and CDPK13 play key roles in acclimation to low oxygen through the control of RBOH-mediated ROS production in rice.Plant physiology · 2024Article
- Post-Translational Modification of WRKY Transcription Factors.Plants (Basel, Switzerland) · 2024Review
- Tomato SlWRKY3 Negatively RegulatesPlants (Basel, Switzerland) · 2024Article
- Activation of MAPK-mediated immunity by phosphatidic acid in response to positive-strand RNA viruses.Plant communications · 2024Article
- Comparative transcriptome analysis reveals nicotine metabolism is a critical component for enhancing stress response intensity of innate immunity system in tobacco.Frontiers in plant science · 2024Article
- Large-scale phosphoproteome analysis in wheat seedling leaves provides evidence for extensive phosphorylation of regulatory proteins during CWMV infection.BMC plant biology · 2023Article
- GmWRKY17-mediated transcriptional regulation of GmDREB1D and GmABA2 controls drought tolerance in soybean.Plant molecular biology · 2023Article
- The glycoside hydrolase 28 member VdEPG1 is a virulence factor of Verticillium dahliae and interacts with the jasmonic acid pathway-related gene GhOPR9.Molecular plant pathology · 2023Article
59 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mitogen-activated protein kinase (MAPK) cascades have pivotal roles in plant innate immunity. However, downstream signaling of plant defense-related MAPKs is not well understood. Here, we provide evidence that the Nicotiana benthamiana WRKY8 transcription factor is a physiological substrate of SIPK, NTF4, and WIPK. Clustered Pro-directed Ser residues (SP cluster), which are conserved in group I WRKY proteins, in the N-terminal region of WRKY8 were phosphorylated by these MAPKs in vitro. Antiphosphopeptide antibodies indicated that Ser residues in the SP cluster of WRKY8 are phosphorylated by SIPK, NTF4, and WIPK in vivo. The interaction of WRKY8 with MAPKs depended on its D domain, which is a MAPK-interacting motif, and this interaction was required for effective phosphorylation of WRKY8 in plants. Phosphorylation of WRKY8 increased its DNA binding activity to the cognate W-box sequence. The phospho-mimicking mutant of WRKY8 showed higher transactivation activity, and its ectopic expression induced defense-related genes, such as 3-hydroxy-3-methylglutaryl CoA reductase 2 and NADP-malic enzyme. By contrast, silencing of WRKY8 decreased the expression of defense-related genes and increased disease susceptibility to the pathogens Phytophthora infestans and Colletotrichum orbiculare. Thus, MAPK-mediated phosphorylation of WRKY8 has an important role in the defense response through activation of downstream genes.
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