ArticleAdvances in physiology education2011
Optogenetics in the teaching laboratory: using channelrhodopsin-2 to study the neural basis of behavior and synaptic physiology in Drosophila.
Article in Advances in physiology education, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Behavioral Assays for Optogenetic Manipulation of Neural Circuits in Drosophila melanogaster.Journal of visualized experiments : JoVE · 2025Article
- Investigating the Neural Control of Social Behavior inJournal of undergraduate neuroscience education : JUNE : a publication of FUN, Faculty for Undergraduate Neuroscience · 2025Article
- Optogenetic control ofJournal of microbiology & biology education · 2024Article
- Article
- A Versatile Semester-Long Course-Based Undergraduate Research Experience using Optogenetics and RNAi to Identify Genes Important for Synapse Function.Journal of undergraduate neuroscience education : JUNE : a publication of FUN, Faculty for Undergraduate Neuroscience · 2023Article
- UsingJournal of undergraduate neuroscience education : JUNE : a publication of FUN, Faculty for Undergraduate Neuroscience · 2023Article
- Mapping Neuroscience in the Field of Education through a Bibliometric Analysis.Brain sciences · 2022Review
- An optogenetics device with smartphone video capture to introduce neurotechnology and systems neuroscience to high school students.PloS one · 2022Article
- An economical and highly adaptable optogenetics system for individual and population-level manipulation of Caenorhabditis elegans.BMC biology · 2021Article
- Pandemic Teaching: Using the Allen Cell Types Database for Final Semester Projects in an Undergraduate Neurophysiology Lab Course.Journal of undergraduate neuroscience education : JUNE : a publication of FUN, Faculty for Undergraduate Neuroscience · 2021Article
- Inexpensive Methods for Live Imaging of Central Pattern Generator Activity in theJournal of undergraduate neuroscience education : JUNE : a publication of FUN, Faculty for Undergraduate Neuroscience · 2020Article
- Building Your Own Neuroscience Equipment: A Precision Micromanipulator and an Epi-fluorescence Microscope for Calcium Imaging.Journal of undergraduate neuroscience education : JUNE : a publication of FUN, Faculty for Undergraduate Neuroscience · 2020Article
- Reducing the Cost of Electrophysiology in the Teaching Laboratory.Journal of undergraduate neuroscience education : JUNE : a publication of FUN, Faculty for Undergraduate Neuroscience · 2018Article
- Probing Synaptic Transmission and Behavior inJournal of undergraduate neuroscience education : JUNE : a publication of FUN, Faculty for Undergraduate Neuroscience · 2018Article
- Aversive and Appetitive Learning inJournal of undergraduate neuroscience education : JUNE : a publication of FUN, Faculty for Undergraduate Neuroscience · 2018Article
- Using Optogenetics to Understand Neuronal Mechanisms Underlying Behavior inJournal of undergraduate neuroscience education : JUNE : a publication of FUN, Faculty for Undergraduate Neuroscience · 2018Article
- Investigation of Seizure-Susceptibility in aGenetics · 2017Article
- Optogenetic Rescue of Locomotor Dysfunction and Dopaminergic Degeneration Caused by Alpha-Synuclein and EKO Genes.Experimental neurobiology · 2017Article
- Grasshopper DCMD: An Undergraduate Electrophysiology Lab for Investigating Single-Unit Responses to Behaviorally-Relevant Stimuli.Journal of undergraduate neuroscience education : JUNE : a publication of FUN, Faculty for Undergraduate Neuroscience · 2017Article
- A Protocol Demonstrating 60 DifferentJournal of undergraduate neuroscience education : JUNE : a publication of FUN, Faculty for Undergraduate Neuroscience · 2017Article
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4 authors.
Funding
Abstract
Here we incorporate recent advances in Drosophila neurogenetics and "optogenetics" into neuroscience laboratory exercises. We used the light-activated ion channel channelrhodopsin-2 (ChR2) and tissue-specific genetic expression techniques to study the neural basis of behavior in Drosophila larvae. We designed and implemented exercises using inexpensive, easy-to-use systems for delivering blue light pulses with fine temporal control. Students first examined the behavioral effects of activating glutamatergic neurons in Drosophila larvae and then recorded excitatory junctional potentials (EJPs) mediated by ChR2 activation at the larval neuromuscular junction (NMJ). Comparison of electrically and light-evoked EJPs demonstrates that the amplitudes and time courses of light-evoked EJPs are not significantly different from those generated by electrical nerve stimulation. These exercises introduce students to new genetic technology for remotely manipulating neural activity, and they simplify the process of recording EJPs at the Drosophila larval NMJ. Relatively little research work has been done using ChR2 in Drosophila, so students have opportunities to test novel hypotheses and make tangible contributions to the scientific record. Qualitative and quantitative assessment of student experiences suggest that these exercises help convey principles of synaptic transmission while also promoting integrative and inquiry-based studies of genetics, cellular physiology, and animal behavior.
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