ArticleThe Journal of biological chemistry2011
Structurally distinct LewisX glycans distinguish subpopulations of neural stem/progenitor cells.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- SSEA-1 Correlates With the Invasive Phenotype in Breast Cancer.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2023Article
- Low-density lipoprotein receptor-related protein-1 (LRP1) in the glial lineage modulates neuronal excitability.Frontiers in network physiology · 2023Review
- Article
- Glycoconjugate journal special issue on: the glycobiology of Parkinson's disease.Glycoconjugate journal · 2022Review
- Complete spatial characterisation of N-glycosylation upon striatal neuroinflammation in the rodent brain.Journal of neuroinflammation · 2021Article
- Neural glycomics: the sweet side of nervous system functions.Cellular and molecular life sciences : CMLS · 2021Review
- Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) as a Novel Regulator of Early Astroglial Differentiation.Frontiers in cellular neuroscience · 2021Article
- Functional characterization of Schistosoma mansoni fucosyltransferases in Nicotiana benthamiana plants.Scientific reports · 2020Article
- Deletion of LRP1 From Astrocytes Modifies Neuronal Network Activity in anFrontiers in cellular neuroscience · 2020Article
- Laser Lesion in the Mouse Visual Cortex Induces a Stem Cell Niche-Like Extracellular Matrix, Produced by Immature Astrocytes.Frontiers in cellular neuroscience · 2020Article
- The distribution of the proteoglycan FORSE-1 in the developing mouse central nervous system.Journal of anatomy · 2019Article
- Synthesis of LewisCarbohydrate research · 2017Article
- Article
- Glycomics: revealing the dynamic ecology and evolution of sugar molecules.Journal of proteomics · 2016Review
- A LewisX glycoprotein screen identifies the low density lipoprotein receptor-related protein 1 (LRP1) as a modulator of oligodendrogenesis in mice.The Journal of biological chemistry · 2013Article
- Transplantation of CD15-enriched murine neural stem cells increases total engraftment and shifts differentiation toward the oligodendrocyte lineage.Stem cells translational medicine · 2013Article
- Differential expression of micro-heterogeneous LewisX-type glycans in the stem cell compartment of the developing mouse spinal cord.Neurochemical research · 2013Article
- A cell surfaceome map for immunophenotyping and sorting pluripotent stem cells.Molecular & cellular proteomics : MCP · 2012Article
- Lewis X-carrying N-glycans regulate the proliferation of mouse embryonic neural stem cells via the Notch signaling pathway.The Journal of biological chemistry · 2012Article
- Normal sulfation levels regulate spinal cord neural precursor cell proliferation and differentiation.Neural development · 2012Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
There is increasing evidence that the stem and progenitor cell population that builds the central nervous system is very heterogeneous. Stem cell markers with the potential to divide this cell pool into subpopulations with distinct characteristics are sparse. We were looking for new cell type-specific antigens to further subdivide the progenitor pool. Here, we introduce the novel monoclonal antibody clone 5750. We show that it specifically labels cell surfaces of neural stem and progenitor cells. When 5750-expressing cells were isolated by fluorescence-activated cell sorting from embryonic mouse brains, the sorted population showed increased neurosphere forming capacity and multipotency. Neurospheres generated from 5750-positive cells could self-renew and remained multipotent even after prolonged passaging. Carbohydrate binding assays revealed that the 5750 antibody specifically binds to LewisX-related carbohydrates. Interestingly, we found that the LewisX epitope recognized by clone 5750 differs from those detected by other anti-LewisX antibody clones like 487(LeX), SSEA-1(LeX), and MMA(LeX). Our data further reveal that individual anti-LewisX clones can be successfully used to label and deplete different subpopulations of neural cells in vivo and in vitro. In conclusion, we present a new tool for the isolation and characterization of neural subpopulations and provide insights into the complexity of cell surface glycosylation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.