ArticleJournal of cell science2011
Target-specific mechanics of phagocytosis: protrusive neutrophil response to zymosan differs from the uptake of antibody-tagged pathogens.
Article in Journal of cell science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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Who cites it
34 citing papers in PubMed, 60 citations in OpenAlex.
- Two-component macrophage model for active phagocytosis with pseudopod formation.Biophysical journal · 2024Article
- Ninein promotes F-actin cup formation and inward phagosome movement during phagocytosis in macrophages.Molecular biology of the cell · 2024Article
- Staphylococcal Enterotoxin C2 Mutant-Induced Antitumor Immune Response Is Controlled by CDC42/MLC2-Mediated Tumor Cell Stiffness.International journal of molecular sciences · 2023Article
- Purified complement C3b triggers phagocytosis and activation of human neutrophils via complement receptor 1.Scientific reports · 2023Article
- Mechanisms of frustrated phagocytic spreading of human neutrophils on antibody-coated surfaces.Biophysical journal · 2022Article
- Genome-scale CRISPR screening reveals that C3aR signaling is critical for rapid capture of fungi by macrophages.PLoS pathogens · 2022Article
- Integrative experimental/computational approach establishes active cellular protrusion as the primary driving force of phagocytic spreading by immune cells.PLoS computational biology · 2022Article
- Distinct timing of neutrophil spreading and stiffening during phagocytosis.Biophysical journal · 2022Article
- Using blinking optical tweezers to study cell rheology during initial cell-particle contact.Biophysical journal · 2021Article
- The Activation and Regulation of β2 Integrins in Phagocytes and Phagocytosis.Frontiers in immunology · 2021Review
- Article
- Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).European journal of immunology · 2019Article
- Membrane-cytoskeletal crosstalk mediated by myosin-I regulates adhesion turnover during phagocytosis.Nature communications · 2019Article
- Mechanism of drug extrusion by brain endothelial cells via lysosomal drug trapping and disposal by neutrophils.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Mechanistic Understanding of Single-Cell Behavior is Essential for Transformative Advances in Biomedicine.The Yale journal of biology and medicine · 2018Review
- Phagocytosis and oxycytosis: two arms of human innate immunity.Immunologic research · 2018Review
- Extension of chemotactic pseudopods by nonadherent human neutrophils does not require or cause calcium bursts.Science signaling · 2018Article
- Guidelines for the use of flow cytometry and cell sorting in immunological studies.European journal of immunology · 2017Article
- Multiple roles of filopodial dynamics in particle capture and phagocytosis and phenotypes of Cdc42 and Myo10 deletion.The Journal of biological chemistry · 2017Article
- Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The physical mechanisms that control target-specific responses of human neutrophils to distinct immune threats are poorly understood. Using dual-micropipette manipulation, we have quantified and compared the time courses of neutrophil phagocytosis of two different targets: zymosan (a prominent model of fungal infection), and antibody-coated (Fc) particles. Our single-live-cell/single-target approach exposes surprising differences between these two forms of phagocytosis. Unlike the efficient uptake of 3-μm Fc targets (within ~66 seconds), the engulfment of similarly sized zymosan is slow (~167 seconds), mainly due to the formation of a characteristic pedestal that initially pushes the particle outwards by ~1 μm. Despite a roughly twofold difference in maximum cortical tensions, the top 'pull-in' speeds of zymosan and Fc targets are indistinguishable at ~33 nm/second. Drug inhibition shows that both actin as well as myosin II partake in the regulation of neutrophil cortical tension and cytoplasmic viscosity; other than that, myosin II appears to play a minor role in both forms of phagocytosis. Remarkably, an intact actin cytoskeleton is required to suppress, in antibody-mediated phagocytosis, the initially protrusive deformation that distinguishes the neutrophil response to zymosan.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.